Application | Comment | Organism |
---|---|---|
biotechnology | Amycolatopsis sp. ATCC 39116 vdh mutant represents an optimized and industrially applicable platform for biotechnological production of natural vanillin | Amycolatopsis sp. |
Cloned (Comment) | Organism |
---|---|
vdh gene cloned into pET23a(+)vector heterologously expressed with C-terminal His6-tag in Escherichia coli BL21(DE3) cells | Amycolatopsis sp. |
General Stability | Organism |
---|---|
after storage at 4°C for 24 h, the enzyme exhibits 86% of its former activity | Amycolatopsis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
SDS-PAGE | Amycolatopsis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis sp. | K9UV87 | - |
- |
Purification (Comment) | Organism |
---|---|
purified to apparent electrophoretic homogeneity using His SpinTrap columns | Amycolatopsis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no VDH activity detectable in cells that are cultivated with gluconate as the sole carbon source | Amycolatopsis sp. |
0.025 | - |
crude extract, when cells are previously cultivated with vanillin as the carbon source, pH 7.1 and 30°C | Amycolatopsis sp. |
0.072 | - |
purified recombinant protein, low activity due to loss of activity during purification, pH 7.1 and 30°C | Amycolatopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | 66% activity in comparison to vanillin | Amycolatopsis sp. | benzoate + NADH + 2 H+ | - |
? | |
cinnamaldehyde + NAD+ + H2O | - |
Amycolatopsis sp. | cinnamate + NADH + 2 H+ | - |
? | |
coniferylaldehyde + NAD+ + H2O | - |
Amycolatopsis sp. | coniferic acid + NADH + 2 H+ | - |
? | |
additional information | vdh deletion mutant loses its ability to grow on vanillin and does not show vanillin dehydrogenase activity, 2.3fold higher vanillin concentration in vdh mutants observed when ferulic acid is provided for biotransformation in a cultivation experiment | Amycolatopsis sp. | ? | - |
? | |
vanillin + NAD+ + H2O | no enzyme activity observed in the absence of NAD+, no oxidation of vanillin detectable when NADP+ used as cofactor | Amycolatopsis sp. | vanillate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
vanillin dehydrogenase | - |
Amycolatopsis sp. |
VDH | - |
Amycolatopsis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
- |
Amycolatopsis sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 65 | - |
Amycolatopsis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Amycolatopsis sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Amycolatopsis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Amycolatopsis sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | Amycolatopsis sp. ATCC 39116 is capable of synthesizing large amounts of vanillin from the natural substrate ferulic acid | Amycolatopsis sp. |