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Literature summary for 1.2.1.60 extracted from
- Control of meta-cleavage degradation of 4-hydroxyphenylacetate in Pseudomonas putida (1981), J. Bacteriol., 147, 844-850.
General Stability
| General Stability | Organism |
|---|---|
| heating at 50°C for 3 min and immediate chilling on ice abolishes enzyme activity in crude cell extracts | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |  |
| 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Pseudomonas putida NCIB 9865 | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida NCIB 9865 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strict dependency for induction of enzyme synthesis of the meta-cleavage pathway by 4-hydroxyphenylacetate | Pseudomonas putida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
| 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Pseudomonas putida NCIB 9865 | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
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Release 2023.1 (January 2023)
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