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Literature summary for 1.2.1.59 extracted from

  • Schäfer, T.; Schönheit, P.
    Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus involvement of reactions of the Embden-Meyerhof pathway (1993), Arch. Microbiol., 159, 354-363 .
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Search for more literature for 1.2.1.59

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 NADPH 50°C, pH not specified in the publication Pyrococcus furiosus
0.05
-
 NADP+ 50°C, pH not specified in the publication Pyrococcus furiosus
1.4
-
 NADH 50°C, pH not specified in the publication Pyrococcus furiosus
1.6
-
 NAD+ 50°C, pH not specified in the publication Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P61879
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.009
-
 50°C, pH not specified in the publication, cofactor: NAD+, in presence of arsenate Pyrococcus furiosus
0.019
-
 50°C, pH not specified in the publication, cofactor: NADP+, in presence of arsenate Pyrococcus furiosus
0.035
-
 50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH Pyrococcus furiosus
0.06
-
 50°C, pH not specified in the publication, reduction of 1,3-phosphoglycerate with NADPH Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrococcus furiosus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
D-glyceraldehyde 3-phosphate + phosphate + NADP+ NADP+ is the preferred cofactor Pyrococcus furiosus 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
glyceraldehyde-3-phosphate dehydrogenase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pyrococcus furiosus
NADP+ NADP+ is the preferred cofactor Pyrococcus furiosus

Expression

Organism Comment Expression
Pyrococcus furiosus induced 10fold under growth conditions which require gluconeogenesis up