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Literature summary for 1.2.1.59 extracted from

  • Zwickl, P.; Fabry, S.; Bogedain, C.; Haas, A.; Hensel, R.
    Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli (1990), J. Bacteriol., 172, 4329-4338.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression at high levels in Escherichia coli, the primary structure exhibits a strikingly high proportion of aromatic amino acids Pyrococcus woesei

General Stability

General Stability Organism
several salts stabilize against thermal inactivation, K+-citrate is most effective Pyrococcus woesei

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive to pentalenolactone Pyrococcus woesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADP+
-
 Pyrococcus woesei
1
-
 NAD+
-
 Pyrococcus woesei

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate dependent on Pyrococcus woesei
phosphate Km: 0.04 mM Pyrococcus woesei

Organism

Organism UniProt Comment Textmining
Pyrococcus woesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus woesei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pyrococcus woesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Pyrococcus woesei 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+
-
 Pyrococcus woesei 1,3-diphosphoglycerate + NAD(P)H
-
 ?
D-glyceraldehyde-3-phosphate + phosphate + NADP+
-
 Pyrococcus woesei 1,3-diphosphoglycerate + NADPH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 44 min Pyrococcus woesei