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Literature summary for 1.2.1.48 extracted from

  • Tao, X.; Zhang, Z.; Zhang, X.; Li, H.; Sun, H.; Mao, Z.; Xia, W.
    Structural insight into the substrate gating mechanism by Staphylococcus aureus aldehyde dehydrogenase (2020), CCS Chem., 2, 946-954 .
No PubMed abstract available
Search for more literature for 1.2.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
apo- and holoenzyme forms of mutant C244S, sitting drop vapor diffusion method, using 40% (w/v) PEG400, 200 mM MgSO4, 100 mM MES, pH 5.5 for the apoenzyme and 1 M Li2SO4, 100 mM cacodylate trihydrate, pH 6.0 for the holoenzyme Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C244S the mutant shows about 90% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus
F456A the mutant shows about 50% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus
F456A/F457A the mutant shows about 15% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus
F457A the mutant shows about 40% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus
K449E the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus
Y92A the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,4'-diapolycopendial + NAD+ + H2O
-
 Staphylococcus aureus 4,4'-diapolycopen-4'-al-4-oic acid + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Staphylococcus aureus benzoate + NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 51700, calculated from amino acid sequence Staphylococcus aureus

Synonyms

Synonyms Comment Organism
ALDH
-
 Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Staphylococcus aureus