Literature summary for 1.2.1.46 extracted from

Ashraf, R.; Rashid, N.; Basheer, S.; Aziz, I.; Akhtar, M.
Glutathione-dependent formaldehyde dehydrogenase homolog from Bacillus subtilis strain R5 is a propanol-preferring alcohol dehydrogenase (2017), Biochemistry, 82, 13-23 .

Search for more literature for 1.2.1.46

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL cells	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	significant inhibition at 10 mM	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	the protein contains 1.3 mol of Cu2+. The highest activity, 22fold increase, is found in the protein produced in the presence of 0.025 mM Cu2+	Bacillus subtilis
Fe2+	a 20fold increase in activity is found in the protein produced in the presence of 0.025 mM Fe2+	Bacillus subtilis
additional information	the presence or absence of metal ions (0.1 mM) in the reaction mixture does not affect the enzyme activity	Bacillus subtilis
Zn2+	the protein contains 1.5 mol of Zn2+. An increase in activity is found in the protein produced in the presence of 0.025 mM Zn2+	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
164000	-	gel filtration	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Bacillus subtilis R5	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel chelate affinity column chromatography and Superdex 200 gel filtration	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetaldehyde + NAD+ + H2O	95% activity compared to propionaldehyde	Bacillus subtilis	acetate + NADH + H+	-	?
formaldehyde + NAD+ + H2O	78% activity compared to propionaldehyde	Bacillus subtilis	formate + NADH + H+	-	?
propionaldehyde + NAD+ + H2O	95% activity compared to propionaldehyde	Bacillus subtilis	propionate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 42000, SDS-PAGE	Bacillus subtilis
homotetramer	4 * 41208, calculated from amino acid sequence	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase	cf. EC 1.1.1.1	Bacillus subtilis
GSH-FDH/ADH	-	Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	65	more than 50% activity between 50 and 65°C	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	pH optimum for reduction reaction in sodium phosphate buffer using acetaldehyde as the substrate	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
glutathione	dependent on	Bacillus subtilis
NAD+	-	Bacillus subtilis

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Release 2023.1 (January 2023)