Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.44 extracted from

  • Chao, N.; Li, S.; Li, N.; Qi, Q.; Jiang, W.T.; Jiang, X.N.; Gai, Y.
    Two distinct cinnamoyl-CoA reductases in Selaginella moellendorffii offer insight into the divergence of CCRs in plants (2017), Planta, 246, 33-43 .
    View publication on PubMed
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Selaginella moellendorffii

Protein Variants

Protein Variants Comment Organism
S123T the mutant shows reduced activity compared to the wild type enzyme Selaginella moellendorffii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02759
-
 feruloyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.05092
-
 sinapoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.05581
-
 4-coumaroyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.11608
-
 4-coumaroyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.13699
-
 feruloyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.15233
-
 caffeoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.28301
-
 caffeoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.56836
-
 sinapoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii

Organism

Organism UniProt Comment Textmining
Selaginella moellendorffii
-
-
-
Selaginella moellendorffii A0A1V0HSA2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Selaginella moellendorffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+
-
 Selaginella moellendorffii 4-coumaraldehyde + CoA + NADP+
-
 ?
caffeoyl-CoA + NADPH + H+
-
 Selaginella moellendorffii caffeylaldehyde + NADP+
-
 ?
feruloyl-CoA + NADPH + H+
-
 Selaginella moellendorffii coniferaldehyde + CoA + NADP+
-
 ?
feruloyl-CoA + NADPH + H+ preferred substrate of isoform CCR1 Selaginella moellendorffii coniferaldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+
-
 Selaginella moellendorffii sinapaldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+ preferred substrate of isoform CCR2-1 Selaginella moellendorffii sinapaldehyde + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Selaginella moellendorffii

Synonyms

Synonyms Comment Organism
CCR1 isoform Selaginella moellendorffii
CCR2-1 isoform Selaginella moellendorffii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Selaginella moellendorffii
50
-
-
 Selaginella moellendorffii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 the enzyme activity is optimal at 30°C, with a steep decrease when the reaction is performed at 40°C. Isoform CCR1 exhibits relatively high activity at low temperature Selaginella moellendorffii
10 60 the enzyme shows approximately 50% activity at 60°C, approximately 5.3% activity at 20°C and no activity at 10°C Selaginella moellendorffii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
 4-coumaroyl-CoA mutant S123T of isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.052
-
 sinapoyl-CoA mutant S123T of isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.093
-
 caffeoyl-CoA mutant S123T of isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.136
-
 feruloyl-CoA mutant S123T of isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.232
-
 4-coumaroyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.24
-
 feruloyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.261
-
 sinapoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.289
-
 feruloyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.324
-
 4-coumaroyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.326
-
 caffeoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
0.559
-
 caffeoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
0.829
-
 sinapoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Selaginella moellendorffii
6.5 7
-
 Selaginella moellendorffii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Selaginella moellendorffii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
 sinapoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
1.8
-
 feruloyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
2
-
 caffeoyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
2
-
 4-coumaroyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
2.2
-
 caffeoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
4.3
-
 4-coumaroyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii
5.2
-
 sinapoyl-CoA isoform CCR2-1, pH and temperature not specified in the publication Selaginella moellendorffii
10.5
-
 feruloyl-CoA isoform CCR1, pH and temperature not specified in the publication Selaginella moellendorffii