Cloned (Comment) | Organism |
---|---|
gene SbCCR1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis | Sorghum bicolor |
gene SbCCR2-1, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis | Sorghum bicolor |
gene SbCCR2-2, DNA and amino acid sequence determination and analysis, cloning and expression in Escherichia coli strain DH5alpha, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthaminana leaves via Agrobacterum tumefaciens transfection, quantitative real-time PCR enzyme expression analysis | Sorghum bicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sorghum bicolor | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
- |
- |
Sorghum bicolor | C5XWV7 | - |
- |
Sorghum bicolor | C5YLL4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Sorghum bicolor | - |
additional information | the highest expression levels of the three CCR genes occur in stem. The expression levels of SbCCR1 and SbCCR2-2 in stem and root are higher than in spikelet and leaf, but those of SbCCR2-1 in stem and spikelet are higher than in leaf and root. The gene copy number of SbCCR1 is significantly higher than those of SbCCR2-1 and SbCCR2-2 in root, leaf, stem and spikelet, especially in stem | Sorghum bicolor | - |
root | - |
Sorghum bicolor | - |
spikelet | - |
Sorghum bicolor | - |
stem | - |
Sorghum bicolor | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 37950, about, sequence calculation | Sorghum bicolor |
? | x * 38210, about, sequence calculation | Sorghum bicolor |
? | x * 40240, about, sequence calculation | Sorghum bicolor |
More | the enzyme contains an NADP binding domain, an NWYCY domain, and a substrate domain | Sorghum bicolor |
Synonyms | Comment | Organism |
---|---|---|
CCR | - |
Sorghum bicolor |
SbCCR1 | - |
Sorghum bicolor |
SbCCR2-1 | - |
Sorghum bicolor |
SbCCR2-2 | - |
Sorghum bicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sorghum bicolor | sequence calculation | - |
5.47 |
Sorghum bicolor | sequence calculation | - |
6.5 |
Sorghum bicolor | sequence calculation | - |
8.79 |
Organism | Comment | Expression |
---|---|---|
Sorghum bicolor | the enzyme is induced by drought treatment | up |
Sorghum bicolor | the enzyme is induced by drought treatment, genes SbCCR1 and SbCCR2-2 mainly respond in the first phase of drought defense, while the SbCCR2-1 gene responds during the whole drought defense period | up |
General Information | Comment | Organism |
---|---|---|
metabolism | cinnamoyl-CoA reductase (CCR) is the first enzyme in the monolignol-specific branch of the lignin biosynthetic pathway | Sorghum bicolor |
physiological function | the enzyme is involved in drought defense | Sorghum bicolor |