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Literature summary for 1.2.1.44 extracted from

  • Han, B.; Liu, S.; Gai, Y.; Jiang, X.
    Preparation of cinnamoyl-CoA and analysis of the enzyme activity of recombinant CCR protein from Populus tomentosa (2014), J. Plant Biochem. Biotechnol., 23, 348-357 .
No PubMed abstract available
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
CCR, recombinant expression of His-tagged enzyme CCR in Escherichia coli, coexpression with 4-coumarate-CoA ligase, EC 6.2.1.12. The best expression conditions for recombinant PtCCR are 6 h of 0.4 mM IPTG induction at 37°C Populus tomentosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics analysis Populus tomentosa
0.323
-
 feruloyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa
0.377
-
 4-coumaroyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa
0.617
-
 caffeoyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+ Populus tomentosa
-
 4-coumaraldehyde + CoA + NADP+
-
 ?
caffeoyl-CoA + NADPH + H+ Populus tomentosa
-
 caffealdehyde + CoA + NADP+
-
 ?
cinnamoyl-CoA + NADPH + H+ Populus tomentosa
-
 cinnamaldehyde + CoA + NADP+
-
 ?
feruloyl-CoA + NADPH + H+ Populus tomentosa preferred substrate coniferaldehyde + CoA + NADP+
-
 ?
sinapoyl-CoA + NADPH + H+ Populus tomentosa
-
 sinapaldehyde + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Populus tomentosa Q6S3D7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme CCR from Escherichia coli by nickel affinity chromatography and gel filtration Populus tomentosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+
-
 Populus tomentosa 4-coumaraldehyde + CoA + NADP+
-
 ?
caffeoyl-CoA + NADPH + H+
-
 Populus tomentosa caffealdehyde + CoA + NADP+
-
 ?
cinnamoyl-CoA + NADPH + H+
-
 Populus tomentosa cinnamaldehyde + CoA + NADP+
-
 ?
feruloyl-CoA + NADPH + H+ preferred substrate Populus tomentosa coniferaldehyde + CoA + NADP+
-
 ?
additional information the substrates of CCR, cinnamoyl-CoA esters, are products of 4-coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. Recombinant Pt4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency Populus tomentosa ?
-
 ?
sinapoyl-CoA + NADPH + H+
-
 Populus tomentosa sinapaldehyde + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, recombinant His-tagged enzyme CCR, SDS-PAGE Populus tomentosa

Synonyms

Synonyms Comment Organism
CCR
-
 Populus tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Populus tomentosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.25
-
 assay at Populus tomentosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.9 activity range, inactive above pH 7.0, profile overview Populus tomentosa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Populus tomentosa

General Information

General Information Comment Organism
physiological function cinnamoyl-CoA reductase catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes and is considered as a key enzyme in lignin formation Populus tomentosa