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Literature summary for 1.2.1.44 extracted from

  • Yeh, S.Y.; Huang, F.C.; Hoffmann, T.; Mayershofer, M.; Schwab, W.
    FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.) (2014), Front. Plant Sci., 5, 518 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.44

Cloned(Commentary)

Cloned (Comment) Organism
gene FaCCR, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Fragaria x ananassa

Protein Variants

Protein Variants Comment Organism
additional information gene expression level and enzymatic activity of FaCCR are efficiently suppressed through RNAi in FaCCR-silenced strawberries. Levels of G-monomers are considerably reduced in FaCCR-silenced fruits. The ihpRNA-FaCCR construct shows sequence-specific interference with homolo-gous FaCCR expression in the fruits, phenotype, overview Fragaria x ananassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Fragaria x ananassa
0.0161
-
 feruloyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.0248
-
 4-coumaroyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.0255
-
 caffeoyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+ Fragaria x ananassa
-
 4-coumaraldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+ Fragaria x ananassa
-
 caffealdehyde + CoA + NADP+
-
 r
cinnamoyl-CoA + NADPH + H+ Fragaria x ananassa
-
 cinnamaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ Fragaria x ananassa
-
 coniferaldehyde + CoA + NADP+
-
 r
sinapoyl-CoA + NADPH + H+ Fragaria x ananassa
-
 sinapaldehyde + CoA + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa J7II91 Fragaria chiloensis x Fragaria virginiana, cv. Elsanta
-

Source Tissue

Source Tissue Comment Organism Textmining
flower very low enzyme expression Fragaria x ananassa
-
fruit strong enzyme expression in turning and red ripe fruits. The CCR gene shows higher expression levels in varieties that produce fruits with soft tissue Fragaria x ananassa
-
leaf very low enzyme expression Fragaria x ananassa
-
additional information relative expression profiles of monolignol biosynthesis genes in vegetative tissues, flowers, and fruit developmental stages of Fragaria ananassa cv.Elsanta Fragaria x ananassa
-
root low enzyme expression Fragaria x ananassa
-
runner low enzyme expression Fragaria x ananassa
-
stem low enzyme expression Fragaria x ananassa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + NADPH + H+
-
 Fragaria x ananassa 4-coumaraldehyde + CoA + NADP+
-
 r
4-coumaroyl-CoA + NADPH + H+ low activity Fragaria x ananassa 4-coumaraldehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+
-
 Fragaria x ananassa caffealdehyde + CoA + NADP+
-
 r
caffeoyl-CoA + NADPH + H+ low activity Fragaria x ananassa caffealdehyde + CoA + NADP+
-
 r
cinnamoyl-CoA + NADPH + H+
-
 Fragaria x ananassa cinnamaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+
-
 Fragaria x ananassa coniferaldehyde + CoA + NADP+
-
 r
feruloyl-CoA + NADPH + H+ highly preferred substrate Fragaria x ananassa coniferaldehyde + CoA + NADP+
-
 r
additional information enzymatic assays with aGST-FaCCR reaction containing a mixture of equal molar amounts of three substrates (caffeoyl-, 4-coumaroyl-, and feruloyl-CoA), yield three major peaks that are identified as caffeic aldehyde (3,4-dihydroxycinnamaldehyde), 4-coumaraldehyde, and coniferaldehyde, respectively Fragaria x ananassa ?
-
 ?
sinapoyl-CoA + NADPH + H+
-
 Fragaria x ananassa sinapaldehyde + CoA + NADP+
-
 r

Synonyms

Synonyms Comment Organism
CCR
-
 Fragaria x ananassa
cinnamoyl-CoA reductase
-
 Fragaria x ananassa
FaCCR
-
 Fragaria x ananassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Fragaria x ananassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000265
-
 4-coumaroyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.0000293
-
 caffeoyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.00234
-
 feruloyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Fragaria x ananassa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Fragaria x ananassa
NADPH
-
 Fragaria x ananassa

Expression

Organism Comment Expression
Fragaria x ananassa no significant induction of FaCCR gene in the fruits exposed to Agrobacterium, ascompared to control fruits during 4 days. The expression level can even be reduced upon agroinfiltration additional information

General Information

General Information Comment Organism
malfunction levels of G-monomers are considerably reduced in FaCCR-silenced fruits. Phenotypic analysis shows that the texture of the fruits injected with different FaCCR constructs is more solid than that of the untreated fruits , but up- or downregulation of the enzyme does not alter the red color and appearance of the fruits Fragaria x ananassa
metabolism the enzyme is involved in the lignin biosynthesis pathway Fragaria x ananassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00107
-
 4-coumaroyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.00115
-
 caffeoyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa
0.145
-
 feruloyl-CoA pH 6.0, 25°C, recombinant GST-tagged enzyme Fragaria x ananassa