Cloned (Comment) | Organism |
---|---|
into the pET41a+ and pET23b+ vector for expression in Escherichia coli BL21DE3 cells | Populus tremuloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxyferuloyl-CoA | competitive inhibitor | Populus tremuloides | |
caffeoyl-CoA | competitive inhibitor | Populus tremuloides | |
feruloyl-CoA | competitive inhibitor | Populus tremuloides | |
sinapoyl-CoA | competitive inhibitor | Populus tremuloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0137 | - |
feruloyl-CoA | - |
Populus tremuloides | |
0.0763 | - |
caffeoyl-CoA | - |
Populus tremuloides | |
0.099 | - |
5-hydroxyferuloyl-CoA | - |
Populus tremuloides | |
0.1045 | - |
sinapoyl-CoA | - |
Populus tremuloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cinnamaldehyde + CoA + NADP+ | Populus tremuloides | - |
cinnamoyl-CoA + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremuloides | Q9M631 | - |
- |
Purification (Comment) | Organism |
---|---|
CCR protein is purified using a Bug-Buster GSTclosed circleBind purification kit, and the fused tag is cleaved and removed using the Enterokinase Cleavage capture kit | Populus tremuloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
xylem | - |
Populus tremuloides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
feruloyl-CoA as a substrate at pH 7.0 | Populus tremuloides |
0.02 | - |
feruloyl-CoA as a substrate at pH 6.5 | Populus tremuloides |
0.1 | - |
feruloyl-CoA as a substrate at pH 5.0 | Populus tremuloides |
0.12 | - |
feruloyl-CoA as a substrate at pH 5.5 | Populus tremuloides |
0.14 | - |
feruloyl-CoA as a substrate at pH 6.0 | Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyferuloyl-CoA + NADPH + H+ | - |
Populus tremuloides | 5-hydroxyconiferaldehyde + CoA + NADP+ | - |
? | |
caffeoyl-CoA + NADPH + H+ | - |
Populus tremuloides | caffealdehyde + CoA + NADP+ | - |
? | |
cinnamaldehyde + CoA + NADP+ | - |
Populus tremuloides | cinnamoyl-CoA + NADPH + H+ | - |
? | |
feruloyl-CoA + NADPH + H+ | - |
Populus tremuloides | coniferaldehyde + CoA + NADP+ | - |
? | |
p-coumaroyl-CoA + NADPH + H+ | - |
Populus tremuloides | p-coumaraldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH + H+ | - |
Populus tremuloides | sinapaldehyde + CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCR | ambiguous | Populus tremuloides |
cinnamoyl-Co-enzyme A reductase | - |
Populus tremuloides |
cinnamoyl-CoA reductase | - |
Populus tremuloides |
PtCCR | - |
Populus tremuloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
caffeoyl-CoA | - |
Populus tremuloides | |
4.9 | - |
5-hydroxyferuloyl-CoA | - |
Populus tremuloides | |
5.3 | - |
sinapoyl-CoA | - |
Populus tremuloides | |
7.8 | - |
feruloyl-CoA | - |
Populus tremuloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
activity assay, feruloyl-CoA as substrate | Populus tremuloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | when the pH is above 6.5, the activity becomes negligible, feruloyl-CoA as substrate | Populus tremuloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Populus tremuloides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
feruloyl-CoA | competitive inhibition of feruloyl-CoA on the 5-hydroxyferuloyl-CoA reaction | Populus tremuloides | |
0.0066 | - |
feruloyl-CoA | competitive inhibition of feruloyl-CoA on the sinapoyl-CoA reaction | Populus tremuloides | |
0.0083 | - |
feruloyl-CoA | competitive inhibition of feruloyl-CoA on the caffeoyl-CoA reaction | Populus tremuloides | |
0.0153 | - |
caffeoyl-CoA | competitive inhibition of caffeoyl-CoA on the feruloyl-CoA reaction | Populus tremuloides | |
0.0157 | - |
5-hydroxyferuloyl-CoA | competitive inhibition of 5-hydroxyferuloyl-CoA on the feruloyl-CoA reaction | Populus tremuloides | |
0.02 | - |
sinapoyl-CoA | competitive inhibition of sinapoyl-CoA on the feruloyl-CoA reaction | Populus tremuloides |