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Literature summary for 1.2.1.44 extracted from
- Enzymic synthesis of lignin precursors. Comparison of cinnamoyl-CoA reductase and cinnamyl alcohol:NADP+ dehydrogenase from spruce (Picea abies L.) and soybean (Glycine max L.) (1981), Eur. J. Biochem., 119, 115-124.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol: stabilizes , only stable in presence of | Glycine max |
| 2-mercaptoethanol: stabilizes , only stable in presence of | Picea abies |
| ethyleneglycol, 15%, stabilizes | Glycine max |
| ethyleneglycol, 15%, stabilizes | Picea abies |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Glycine max | |
| additional information | - |
additional information | - |
Picea abies |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | of cambial cells | Picea abies | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
gel filtration | Picea abies |
| 36000 | - |
SDS slab-gel electrophoresis | Glycine max |
| 38700 | - |
1 * 38700, SDS-PAGE | Picea abies |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
L. var. Mandarin, soybean | - |
| Picea abies | - |
L. Karst, spruce | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
- |
Picea abies |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cambium | cambium sap, mainly from cambial and young xylem cells | Picea abies | - |
| cell culture | - |
Glycine max | - |
| hypocotyl | - |
Picea abies | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.15 | - |
- |
Picea abies |
| 84.07 | - |
- |
Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, presence of: NADP+, 2-mercaptoethanol, ethyleneglycol, several months | Picea abies |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cinnamoyl-CoA + NADPH | - |
Glycine max | cinnamaldehyde + CoA + NADP+ | - |
? |  |
| cinnamoyl-CoA + NADPH | - |
Picea abies | cinnamaldehyde + CoA + NADP+ | - |
? | |
| feruloyl-CoA + NADPH | - |
Glycine max | ferulic aldehyde + CoA + NADP+ | - |
r | |
| feruloyl-CoA + NADPH | - |
Picea abies | ferulic aldehyde + CoA + NADP+ | - |
r | |
| p-coumaroyl-CoA + NADPH | i.e. p-hydroxycinnamoyl-CoA, poor substrate | Glycine max | p-coumaric aldehyde + CoA + NADP+ | - |
? | |
| p-coumaroyl-CoA + NADPH | i.e. p-hydroxycinnamoyl-CoA, poor substrate | Picea abies | p-coumaric aldehyde + CoA + NADP+ | - |
? | |
| sinapoyl-CoA + NADPH | - |
Glycine max | sinapic aldehyde + CoA + NADP+ | - |
? | |
| sinapoyl-CoA + NADPH | - |
Picea abies | sinapic aldehyde + CoA + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000 SDS-PAGE | Glycine max |
| monomer | 1 * 38700, SDS-PAGE | Picea abies |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Picea abies |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Glycine max | |
| NADP+ | - |
Picea abies | |
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