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Literature summary for 1.2.1.42 extracted from

  • Willis, R.M.; Wahlen, B.D.; Seefeldt, L.C.; Barney, B.M.
    Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol (2011), Biochemistry, 50, 10550-10558.
    View publication on PubMed
Search for more literature for 1.2.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TB1 Marinobacter nauticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 hexadecanoyl-CoA at pH 7.0 and 37°C Marinobacter nauticus
0.05
-
 cis-11-hexadecenal at pH 7.0 and 37°C Marinobacter nauticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 x * 116000, SDS-PAGE Marinobacter nauticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonoyl-CoA + NADPH + H+ Marinobacter nauticus 86% activity compared to hexadecanoyl-CoA arachidonic aldehyde + CoA + NADP+
-
 ?
cis-11-hexadecenal + CoA + NADP+ Marinobacter nauticus
-
 (E)-hexadec-11-enoyl-CoA + NADPh + H+
-
 ?
dodecanoyl-CoA + NADPH + H+ Marinobacter nauticus 26% activity compared to hexadecanoyl-CoA dodecanal + CoA + NADP+
-
 ?
hexadecanoyl-CoA + NADPH + H+ Marinobacter nauticus 100% activity 1-hexadecanal + CoA + NADP+
-
 ?
additional information Marinobacter nauticus the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal ?
-
 ?
octadecanoyl-CoA + NADPH + H+ Marinobacter nauticus 69% activity compared to hexadecanoyl-CoA octadecanal + CoA + NADP+
-
 ?
octanoyl-CoA + NADPH + H+ Marinobacter nauticus 9% activity compared to hexadecanoyl-CoA octanal + CoA + NADP+
-
 ?
oleoyl-CoA + NADPH + H+ Marinobacter nauticus 78% activity compared to hexadecanoyl-CoA oleic aldehyde + CoA + NADP+
-
 ?
palmitoleoyl-CoA + NADPH + H+ Marinobacter nauticus 99% activity compared to hexadecanoyl-CoA palmitoleic aldehyde + CoA + NADP+
-
 ?
tetradecanoyl-CoA + NADPH + H+ Marinobacter nauticus 60% activity compared to hexadecanoyl-CoA tetradecanal + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Marinobacter nauticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration Marinobacter nauticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 using octanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.034
-
 using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.04
-
 using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.044
-
 using oleoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.049
-
 using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.057
-
 using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
0.057
-
 using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + NADPH + H+ 86% activity compared to hexadecanoyl-CoA Marinobacter nauticus arachidonic aldehyde + CoA + NADP+
-
 ?
cis-11-hexadecenal + CoA + NADP+
-
 Marinobacter nauticus (E)-hexadec-11-enoyl-CoA + NADPh + H+
-
 ?
dodecanoyl-CoA + NADPH + H+ 26% activity compared to hexadecanoyl-CoA Marinobacter nauticus dodecanal + CoA + NADP+
-
 ?
hexadecanoyl-CoA + NADPH + H+ 100% activity Marinobacter nauticus 1-hexadecanal + CoA + NADP+
-
 ?
additional information the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal Marinobacter nauticus ?
-
 ?
octadecanoyl-CoA + NADPH + H+ 69% activity compared to hexadecanoyl-CoA Marinobacter nauticus octadecanal + CoA + NADP+
-
 ?
octanoyl-CoA + NADPH + H+ 9% activity compared to hexadecanoyl-CoA Marinobacter nauticus octanal + CoA + NADP+
-
 ?
oleoyl-CoA + NADPH + H+ 78% activity compared to hexadecanoyl-CoA Marinobacter nauticus oleic aldehyde + CoA + NADP+
-
 ?
palmitoleoyl-CoA + NADPH + H+ 99% activity compared to hexadecanoyl-CoA Marinobacter nauticus palmitoleic aldehyde + CoA + NADP+
-
 ?
tetradecanoyl-CoA + NADPH + H+ 60% activity compared to hexadecanoyl-CoA Marinobacter nauticus tetradecanal + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 116000, SDS-PAGE Marinobacter nauticus

Synonyms

Synonyms Comment Organism
FACoAR
-
 Marinobacter nauticus
fatty acyl-CoA reductase
-
 Marinobacter nauticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Marinobacter nauticus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0 Marinobacter nauticus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Marinobacter nauticus