Any feedback?
Literature summary for 1.2.1.4 extracted from
- Mitochondrial NAD dependent aldehyde dehydrogenase either from yeast or human replaces yeast cytoplasmic NADP dependent aldehyde dehydrogenase for the aerobic growth of yeast on ethanol (2013), Biochim. Biophys. Acta, 1830, 3391-3398.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A200E | the mutant shows strongly reduced activity (50fold) with NADP+ compared to the wild type enzyme | Saccharomyces cerevisiae |
| A200E/V210Q | the mutant shows strongly reduced activity (250fold) with NADP+ and about 3fold increased activity with NAD+ compared to the wild type enzyme | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADP+ | Saccharomyces cerevisiae | - |
acetate + NADPH | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00312 | - |
native enzyme, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
| 0.00315 | - |
mutant enzyme A200E, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
| 0.0113 | - |
mutant enzyme A200E/V210Q, with NAD+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
| 0.012 | - |
mutant enzyme A200E/V210Q, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
| 0.06 | - |
mutant enzyme A200E, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
| 3 | - |
native enzyme, with NADP+ as cofactor, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADP+ | - |
Saccharomyces cerevisiae | acetate + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALDH1 | - |
Saccharomyces cerevisiae |
| NADP dependent aldehyde dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | preferred cofactor | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
