BRENDA - Enzyme Database show
show all sequences of 1.2.1.39

Simultaneous involvement of a tungsten-containing aldehydeferredoxin oxidoreductase and a phenylacetaldehyde dehydrogenase in anaerobic phenylalanine metabolism

Debnar-Daumler, C.; Seubert, A.; Schmitt, G.; Heider, J.; J. Bacteriol. 196, 483-492 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ebA4954, recombinant expression of N-terminally Strep-tagged enzyme in Escherichia coli strain DHalpha
Aromatoleum aromaticum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
at high concentrations
Aromatoleum aromaticum
NAD+
cooperative substrate inhibition
Aromatoleum aromaticum
NADP+
cooperative substrate inhibition
Aromatoleum aromaticum
phenylacetaldehyde
the enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition
Aromatoleum aromaticum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis, overview. The enzyme shows cooperative kinetics toward substrate phenylacetaldehyde and considerable substrate inhibition
Aromatoleum aromaticum
0.018
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
0.025
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetaldehyde + NAD+ + H2O
Aromatoleum aromaticum
-
phenylacetate + NADH + 2 H+
-
-
r
phenylacetaldehyde + NAD+ + H2O
Aromatoleum aromaticum EbN1
-
phenylacetate + NADH + 2 H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aromatoleum aromaticum
Q5P171
-
-
Aromatoleum aromaticum EbN1
Q5P171
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 126fold by cross-flow filtration, ultracentrifugation, and anion exchange chromatography, recombinant Strep-tagged enzyme 137fold from Escherichia coli strain DHalpha by affinity chromatography on a Strep-Tactin column
Aromatoleum aromaticum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.102
-
purified native enzyme, with cofactor NADP+, pH 8.5, 28°C
Aromatoleum aromaticum
0.21
-
purified recombinant Strep-tagged enzyme, pH 8.5, 28°C
Aromatoleum aromaticum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview
742786
Aromatoleum aromaticum
?
-
-
-
-
additional information
enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview
742786
Aromatoleum aromaticum EbN1
?
-
-
-
-
phenylacetaldehyde + NAD+ + H2O
-
742786
Aromatoleum aromaticum
phenylacetate + NADH + 2 H+
-
-
-
r
phenylacetaldehyde + NAD+ + H2O
-
742786
Aromatoleum aromaticum EbN1
phenylacetate + NADH + 2 H+
-
-
-
r
phenylacetaldehyde + NADP+ + H2O
-
742786
Aromatoleum aromaticum
phenylacetate + NADPH + 2 H+
-
-
-
r
phenylacetaldehyde + NADP+ + H2O
-
742786
Aromatoleum aromaticum EbN1
phenylacetate + NADPH + 2 H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 53500, recombinant Strep-tagged enzyme, SDS-PAGE
Aromatoleum aromaticum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Aromatoleum aromaticum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
53
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
63
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6.5
assay at, reverse reaction
Aromatoleum aromaticum
8.5
-
assay at, forward reaction
Aromatoleum aromaticum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
enzyme PDH accepts both NAD+ and NADP+ as electron acceptors, but exhibits 86% of its maximal activity with NAD+ but only 5% of its maximal activity with NADP+
Aromatoleum aromaticum
NAD+
-
Aromatoleum aromaticum
NADH
-
Aromatoleum aromaticum
NADP+
-
Aromatoleum aromaticum
NADPH
-
Aromatoleum aromaticum
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.067
-
NADP+
pH 8.5, 28°C, cooperative substrate inhibition
Aromatoleum aromaticum
0.069
-
NAD+
pH 8.5, 28°C, cooperative substrate inhibition
Aromatoleum aromaticum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ebA4954, recombinant expression of N-terminally Strep-tagged enzyme in Escherichia coli strain DHalpha
Aromatoleum aromaticum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
enzyme PDH accepts both NAD+ and NADP+ as electron acceptors, but exhibits 86% of its maximal activity with NAD+ but only 5% of its maximal activity with NADP+
Aromatoleum aromaticum
NAD+
-
Aromatoleum aromaticum
NADH
-
Aromatoleum aromaticum
NADP+
-
Aromatoleum aromaticum
NADPH
-
Aromatoleum aromaticum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
at high concentrations
Aromatoleum aromaticum
NAD+
cooperative substrate inhibition
Aromatoleum aromaticum
NADP+
cooperative substrate inhibition
Aromatoleum aromaticum
phenylacetaldehyde
the enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition
Aromatoleum aromaticum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.067
-
NADP+
pH 8.5, 28°C, cooperative substrate inhibition
Aromatoleum aromaticum
0.069
-
NAD+
pH 8.5, 28°C, cooperative substrate inhibition
Aromatoleum aromaticum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic analysis, overview. The enzyme shows cooperative kinetics toward substrate phenylacetaldehyde and considerable substrate inhibition
Aromatoleum aromaticum
0.018
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
0.025
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetaldehyde + NAD+ + H2O
Aromatoleum aromaticum
-
phenylacetate + NADH + 2 H+
-
-
r
phenylacetaldehyde + NAD+ + H2O
Aromatoleum aromaticum EbN1
-
phenylacetate + NADH + 2 H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 126fold by cross-flow filtration, ultracentrifugation, and anion exchange chromatography, recombinant Strep-tagged enzyme 137fold from Escherichia coli strain DHalpha by affinity chromatography on a Strep-Tactin column
Aromatoleum aromaticum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.102
-
purified native enzyme, with cofactor NADP+, pH 8.5, 28°C
Aromatoleum aromaticum
0.21
-
purified recombinant Strep-tagged enzyme, pH 8.5, 28°C
Aromatoleum aromaticum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview
742786
Aromatoleum aromaticum
?
-
-
-
-
additional information
enzyme is highly specific for phenylacetaldehyde, has cooperative kinetics toward the substrate, and shows considerable substrate inhibition. No activity with benzaldehyde and indole-3-carbaldehyde, substrate specificity, overview
742786
Aromatoleum aromaticum EbN1
?
-
-
-
-
phenylacetaldehyde + NAD+ + H2O
-
742786
Aromatoleum aromaticum
phenylacetate + NADH + 2 H+
-
-
-
r
phenylacetaldehyde + NAD+ + H2O
-
742786
Aromatoleum aromaticum EbN1
phenylacetate + NADH + 2 H+
-
-
-
r
phenylacetaldehyde + NADP+ + H2O
-
742786
Aromatoleum aromaticum
phenylacetate + NADPH + 2 H+
-
-
-
r
phenylacetaldehyde + NADP+ + H2O
-
742786
Aromatoleum aromaticum EbN1
phenylacetate + NADPH + 2 H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 53500, recombinant Strep-tagged enzyme, SDS-PAGE
Aromatoleum aromaticum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Aromatoleum aromaticum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
53
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
63
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6.5
assay at, reverse reaction
Aromatoleum aromaticum
8.5
-
assay at, forward reaction
Aromatoleum aromaticum
General Information
General Information
Commentary
Organism
metabolism
two distinct enzymes are involved in the oxidation of phenylacetaldehyde to phenylacetate, an aldehyde:ferredoxin oxidoreductase (AOR) and a phenylacetaldehyde dehydrogenase (PDH). PDH is the primary enzyme during anaerobic phenylalanine degradation, whereas AOR is not essential for the metabolic pathway and exhibits probably a function as a detoxifying enzyme if high aldehyde concentrations accumulate in the cytoplasm, which would lead to substrate inhibition of PDH
Aromatoleum aromaticum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
two distinct enzymes are involved in the oxidation of phenylacetaldehyde to phenylacetate, an aldehyde:ferredoxin oxidoreductase (AOR) and a phenylacetaldehyde dehydrogenase (PDH). PDH is the primary enzyme during anaerobic phenylalanine degradation, whereas AOR is not essential for the metabolic pathway and exhibits probably a function as a detoxifying enzyme if high aldehyde concentrations accumulate in the cytoplasm, which would lead to substrate inhibition of PDH
Aromatoleum aromaticum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0025
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
0.003
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0025
-
NADP+
pH 8.5, 28°C
Aromatoleum aromaticum
0.003
-
NAD+
pH 8.5, 28°C
Aromatoleum aromaticum
Other publictions for EC 1.2.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
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2
1
2
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5
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1
1
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2
2
1
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1
2
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1
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2
2
1
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1
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-
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3
3
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-
-
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Simultaneous involvement of a ...
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J. Bacteriol.
196
483-492
2014
-
-
1
-
-
-
4
3
-
-
-
2
-
3
-
-
1
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2
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6
1
1
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2
2
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5
2
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1
5
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4
2
3
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2
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1
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2
-
6
1
1
-
-
2
2
-
-
-
-
1
1
-
2
2
724020
Koma
Production of aromatic compoun ...
Escherichia coli
Appl. Environ. Microbiol.
78
6203-6216
2012
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-
-
-
-
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2
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5
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2
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1
1
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725180
Satoh
Engineering of a tyrosol-produ ...
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J. Agric. Food Chem.
60
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-
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2
2
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686425
Arias
Genetic analyses and molecular ...
Pseudomonas putida
Environ. Microbiol.
10
413-432
2008
-
-
1
-
-
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-
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-
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1
2
-
5
-
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1
1
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1
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1
2
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2
2
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1
1
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2
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690567
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76
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-
-
-
-
-
-
14
2
1
4
2
2
-
4
-
-
1
-
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-
2
1
13
1
1
-
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-
1
1
1
1
-
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-
1
-
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14
-
2
1
4
2
2
-
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-
1
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2
1
13
1
1
-
-
-
1
1
1
-
-
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-
670833
Rodriguez-Zavala
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Escherichia coli
Protein Sci.
15
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8
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1
1
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1
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8
1
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9
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8
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1
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8
1
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9
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718
Schneider
Anaerobic metabolism of L-phen ...
Thauera aromatica
Arch. Microbiol.
168
310-320
1997
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4
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390298
Long
Enzymology of oxidation of tro ...
Pseudomonas sp., Pseudomonas sp. AT3
J. Bacteriol.
179
1044-1050
1997
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6
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4
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1
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4
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390299
Ferrandez
Molecular characterization of ...
Escherichia coli
FEBS Lett.
406
23-27
1997
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1
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1
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3
1
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2
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2
1
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1
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1
1
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1
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3
1
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2
1
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390300
Hanlon
2-Phenylethylamine catabolism ...
Escherichia coli
Microbiology
143
513-518
1997
-
-
-
-
-
-
3
3
-
-
1
2
-
3
-
-
1
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1
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5
1
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1
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1
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3
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3
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1
2
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1
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1
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5
1
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390301
O'Connor
The effect of nutrient limitat ...
Pseudomonas putida, Pseudomonas putida CA-3
Appl. Environ. Microbiol.
62
3594-3599
1996
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2
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9
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4
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1
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4
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390293
Hartmans
Bacterial degradation of styre ...
Bacteria, Bacteria S5
Appl. Environ. Microbiol.
56
1347-1351
1990
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390294
Hartmans
Metabolism of styrene oxide an ...
Xanthobacter sp. 124X, Xanthobacter sp.
Appl. Environ. Microbiol.
55
2850-2855
1989
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2
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4
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7426
Van den Tweel
-
Catabolism of DL-alpha-phenylh ...
Flavobacterium sp.
Arch. Microbiol.
149
207-213
1988
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390296
Parrott
2-Phenylethylamine catabolism ...
Escherichia coli
J. Gen. Microbiol.
133
347-351
1987
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390292
Fujioka
-
Metabolism of phenylalanine (A ...
Achromobacter eurydice
Methods Enzymol.
17A
585-596
1970
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3
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7
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5
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7
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