BRENDA - Enzyme Database show
show all sequences of 1.2.1.39

Purification and characterization of aldehyde dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating Brevibacterium sp. KU1309

Hirano, J.; Miyamoto, K.; Ohta, H.; Appl. Microbiol. Biotechnol. 76, 357-363 (2007)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
8-Quinolinol
34% inhibition in the presence of 1 mM
Brevibacterium sp.
Ba2+
19% inhibition in the presence of 1 mM
Brevibacterium sp.
Cu2+
44% inhibition in the presence of 1 mM
Brevibacterium sp.
EDTA
18% inhibition in the presence of 1 mM
Brevibacterium sp.
Hg2+
77% inhibition in the presence of 1 mM
Brevibacterium sp.
hydrozine
51% inhibition in the presence of 1 mM
Brevibacterium sp.
iodoacetamide
completely inhibits in the presence of 1 mM
Brevibacterium sp.
iodoacetate
18% inhibition in the presence of 1 mM
Brevibacterium sp.
N-ethylmaleinimide
completely inhibits in the presence of 1 mM
Brevibacterium sp.
NaN3
25% inhibition in the presence of 1 mM
Brevibacterium sp.
Ni2+
12% inhibition in the presence of 1 mM
Brevibacterium sp.
p-chloromercuribenzoate
completely inhibits in the presence of 1 mM
Brevibacterium sp.
phenylacetaldehyde
high concentration (0.1 mM) inhibits
Brevibacterium sp.
Zn2+
41% inhibition in the presence of 1 mM
Brevibacterium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00124
-
phenylacetaldehyde
-
Brevibacterium sp.
0.116
-
NAD+
-
Brevibacterium sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Brevibacterium sp.
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM stimulates by 53%
Brevibacterium sp.
Mg2+
1 mM stimulates by 42%
Brevibacterium sp.
Mn2+
1 mM stimulates by 17%
Brevibacterium sp.
additional information
is not stimulated by 10 mM KCl
Brevibacterium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
4 * 61000, SDS-PAGE
Brevibacterium sp.
219000
-
gel filtration
Brevibacterium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetaldehyde + NAD+ + H2O
Brevibacterium sp.
-
phenylacetate + NADH + H+
-
-
?
phenylacetaldehyde + NAD+ + H2O
Brevibacterium sp. KU1309
-
phenylacetate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. KU1309
-
-
-
Purification (Commentary)
Commentary
Organism
by gel filtration, 15.9fold with a 0.21% yield
Brevibacterium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.261
-
cell-free extract
Brevibacterium sp.
4.16
-
15.9fold purified enzyme
Brevibacterium sp.
Storage Stability
Storage Stability
Organism
4°C, 10 mM Tris-HCl buffer
Brevibacterium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-naphthaldehyde + NAD+ + H2O
24% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
1-naphthoate + NADH + H+
-
-
-
?
3-phenylpropionaldehyde + NAD+ + H2O
34% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
3-phenylpropionate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
54% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
54% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
benzoate + NADH + H+
-
-
-
?
decanal + NAD+ + H2O
17% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
decanoate + NADH + H+
-
-
-
?
hexanal + NAD+ + H2O
21% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
hexanoate + NADH + H+
-
-
-
?
hexanal + NAD+ + H2O
21% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
hexanoate + NADH + H+
-
-
-
?
octanal + NAD+ + H2O
31% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
octanoate + NADH + H+
-
-
-
?
octanal + NAD+ + H2O
31% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
octanoate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
690567
Brevibacterium sp.
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
best substrate
690567
Brevibacterium sp.
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
690567
Brevibacterium sp. KU1309
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
best substrate
690567
Brevibacterium sp. KU1309
phenylacetate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 61000, SDS-PAGE
Brevibacterium sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Brevibacterium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Brevibacterium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Brevibacterium sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
at pH 8.0, PADH retains 60% of the initial activity
Brevibacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacterium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-Quinolinol
34% inhibition in the presence of 1 mM
Brevibacterium sp.
Ba2+
19% inhibition in the presence of 1 mM
Brevibacterium sp.
Cu2+
44% inhibition in the presence of 1 mM
Brevibacterium sp.
EDTA
18% inhibition in the presence of 1 mM
Brevibacterium sp.
Hg2+
77% inhibition in the presence of 1 mM
Brevibacterium sp.
hydrozine
51% inhibition in the presence of 1 mM
Brevibacterium sp.
iodoacetamide
completely inhibits in the presence of 1 mM
Brevibacterium sp.
iodoacetate
18% inhibition in the presence of 1 mM
Brevibacterium sp.
N-ethylmaleinimide
completely inhibits in the presence of 1 mM
Brevibacterium sp.
NaN3
25% inhibition in the presence of 1 mM
Brevibacterium sp.
Ni2+
12% inhibition in the presence of 1 mM
Brevibacterium sp.
p-chloromercuribenzoate
completely inhibits in the presence of 1 mM
Brevibacterium sp.
phenylacetaldehyde
high concentration (0.1 mM) inhibits
Brevibacterium sp.
Zn2+
41% inhibition in the presence of 1 mM
Brevibacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00124
-
phenylacetaldehyde
-
Brevibacterium sp.
0.116
-
NAD+
-
Brevibacterium sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Brevibacterium sp.
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
1 mM stimulates by 53%
Brevibacterium sp.
Mg2+
1 mM stimulates by 42%
Brevibacterium sp.
Mn2+
1 mM stimulates by 17%
Brevibacterium sp.
additional information
is not stimulated by 10 mM KCl
Brevibacterium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
4 * 61000, SDS-PAGE
Brevibacterium sp.
219000
-
gel filtration
Brevibacterium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phenylacetaldehyde + NAD+ + H2O
Brevibacterium sp.
-
phenylacetate + NADH + H+
-
-
?
phenylacetaldehyde + NAD+ + H2O
Brevibacterium sp. KU1309
-
phenylacetate + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
by gel filtration, 15.9fold with a 0.21% yield
Brevibacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.261
-
cell-free extract
Brevibacterium sp.
4.16
-
15.9fold purified enzyme
Brevibacterium sp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 10 mM Tris-HCl buffer
Brevibacterium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-naphthaldehyde + NAD+ + H2O
24% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
1-naphthoate + NADH + H+
-
-
-
?
3-phenylpropionaldehyde + NAD+ + H2O
34% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
3-phenylpropionate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
54% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
54% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
benzoate + NADH + H+
-
-
-
?
decanal + NAD+ + H2O
17% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
decanoate + NADH + H+
-
-
-
?
hexanal + NAD+ + H2O
21% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
hexanoate + NADH + H+
-
-
-
?
hexanal + NAD+ + H2O
21% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
hexanoate + NADH + H+
-
-
-
?
octanal + NAD+ + H2O
31% activity relative to phenylacetaldehyde
690567
Brevibacterium sp.
octanoate + NADH + H+
-
-
-
?
octanal + NAD+ + H2O
31% activity relative to phenylacetaldehyde
690567
Brevibacterium sp. KU1309
octanoate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
690567
Brevibacterium sp.
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
best substrate
690567
Brevibacterium sp.
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
-
690567
Brevibacterium sp. KU1309
phenylacetate + NADH + H+
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
best substrate
690567
Brevibacterium sp. KU1309
phenylacetate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 61000, SDS-PAGE
Brevibacterium sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Brevibacterium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Brevibacterium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
-
Brevibacterium sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
8
at pH 8.0, PADH retains 60% of the initial activity
Brevibacterium sp.
Other publictions for EC 1.2.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741787
Crabo
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Arch. Biochem. Biophys.
616
47-58
2017
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1
1
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6
2
-
2
1
2
-
5
-
-
1
1
-
-
-
-
2
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
6
-
2
-
2
1
2
-
-
-
1
-
-
-
-
2
2
1
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1
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3
3
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742786
Debnar-Daumler
Simultaneous involvement of a ...
Aromatoleum aromaticum, Aromatoleum aromaticum EbN1
J. Bacteriol.
196
483-492
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-
-
1
-
-
-
4
3
-
-
-
2
-
3
-
-
1
-
-
-
2
-
6
1
1
-
-
2
2
-
-
5
2
-
-
-
-
1
5
-
-
-
-
4
2
3
-
-
-
2
-
-
-
1
-
-
2
-
6
1
1
-
-
2
2
-
-
-
-
1
1
-
2
2
724020
Koma
Production of aromatic compoun ...
Escherichia coli
Appl. Environ. Microbiol.
78
6203-6216
2012
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2
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5
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-
1
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1
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725180
Satoh
Engineering of a tyrosol-produ ...
Escherichia coli
J. Agric. Food Chem.
60
979-984
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
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-
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-
1
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-
1
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-
1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
686425
Arias
Genetic analyses and molecular ...
Pseudomonas putida
Environ. Microbiol.
10
413-432
2008
-
-
1
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690567
Hirano
Purification and characterizat ...
Brevibacterium sp., Brevibacterium sp. KU1309
Appl. Microbiol. Biotechnol.
76
357-363
2007
-
-
-
-
-
-
14
2
1
4
2
2
-
4
-
-
1
-
-
-
2
1
13
1
1
-
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
1
4
2
2
-
-
-
1
-
-
2
1
13
1
1
-
-
-
1
1
1
-
-
-
-
-
-
-
670833
Rodriguez-Zavala
Characterization of E. coli te ...
Escherichia coli
Protein Sci.
15
1387-1396
2006
-
-
-
-
-
-
-
8
-
-
1
1
-
1
-
-
-
-
-
-
-
-
8
1
-
-
-
9
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
8
-
-
1
1
-
-
-
-
-
-
-
-
8
1
-
-
-
9
-
-
-
-
-
-
-
-
-
-
718
Schneider
Anaerobic metabolism of L-phen ...
Thauera aromatica
Arch. Microbiol.
168
310-320
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390298
Long
Enzymology of oxidation of tro ...
Pseudomonas sp., Pseudomonas sp. AT3
J. Bacteriol.
179
1044-1050
1997
-
-
-
-
-
-
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-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390299
Ferrandez
Molecular characterization of ...
Escherichia coli
FEBS Lett.
406
23-27
1997
-
-
1
-
-
-
-
-
1
-
3
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390300
Hanlon
2-Phenylethylamine catabolism ...
Escherichia coli
Microbiology
143
513-518
1997
-
-
-
-
-
-
3
3
-
-
1
2
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
3
-
-
1
2
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390301
O'Connor
The effect of nutrient limitat ...
Pseudomonas putida, Pseudomonas putida CA-3
Appl. Environ. Microbiol.
62
3594-3599
1996
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390293
Hartmans
Bacterial degradation of styre ...
Bacteria, Bacteria S5
Appl. Environ. Microbiol.
56
1347-1351
1990
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390294
Hartmans
Metabolism of styrene oxide an ...
Xanthobacter sp. 124X, Xanthobacter sp.
Appl. Environ. Microbiol.
55
2850-2855
1989
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7426
Van den Tweel
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Catabolism of DL-alpha-phenylh ...
Flavobacterium sp.
Arch. Microbiol.
149
207-213
1988
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390296
Parrott
2-Phenylethylamine catabolism ...
Escherichia coli
J. Gen. Microbiol.
133
347-351
1987
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390292
Fujioka
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Metabolism of phenylalanine (A ...
Achromobacter eurydice
Methods Enzymol.
17A
585-596
1970
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