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Literature summary for 1.2.1.39 extracted from

  • Arias, S.; Olivera, E.R.; Arcos, M.; Naharro, G.; Luengo, J.M.
    Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U (2008), Environ. Microbiol., 10, 413-432.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Expression in Escherichia coli, production of knock out-mutants is carried out by mutagenesis. Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53290
-
-
Pseudomonas putida
53290
-
calculated Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetaldehyde + NAD+ + H2O Pseudomonas putida
-
phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O Pseudomonas putida
-
phenylacetic acid + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida B1N7H3 U strain, although enzymatic activity decreases in knock-out mutants good phenylacetaldehyde dehydrogenase acivity is present in the bacterium, PeaF and PeaG are suggested to replace PeaE
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Analysis of phenylacetaldehyde dehydrogenase activity in cell-free extracts reveals that although enzymatic activity decreases in the peaE knocked-out mutant (20% with respect to the control), good phenylacetaldehyde dehydrogenase activity is present. Another aldehyde dehydrogenase(s) exist(s) that could replace the role played by PeaE Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetaldehyde + NAD+ + H2O
-
Pseudomonas putida phenylacetate + NADH + H+
-
?
phenylacetaldehyde + NAD+ + H2O
-
Pseudomonas putida phenylacetic acid + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
PeaE
-
Pseudomonas putida
PeaE protein
-
Pseudomonas putida
phenylacetaldehyde dehydrogenase
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida