Cloned (Comment) | Organism |
---|---|
Expression in Escherichia coli, production of knock out-mutants is carried out by mutagenesis. | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53290 | - |
- |
Pseudomonas putida |
53290 | - |
calculated | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | Pseudomonas putida | - |
phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | Pseudomonas putida | - |
phenylacetic acid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | B1N7H3 | U strain, although enzymatic activity decreases in knock-out mutants good phenylacetaldehyde dehydrogenase acivity is present in the bacterium, PeaF and PeaG are suggested to replace PeaE | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Analysis of phenylacetaldehyde dehydrogenase activity in cell-free extracts reveals that although enzymatic activity decreases in the peaE knocked-out mutant (20% with respect to the control), good phenylacetaldehyde dehydrogenase activity is present. Another aldehyde dehydrogenase(s) exist(s) that could replace the role played by PeaE | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetaldehyde + NAD+ + H2O | - |
Pseudomonas putida | phenylacetate + NADH + H+ | - |
? | |
phenylacetaldehyde + NAD+ + H2O | - |
Pseudomonas putida | phenylacetic acid + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PeaE | - |
Pseudomonas putida |
PeaE protein | - |
Pseudomonas putida |
phenylacetaldehyde dehydrogenase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |