BRENDA - Enzyme Database
show all sequences of 1.2.1.38

N-Acetylglutamic gamma-semialdehyde dehydrogenase (Escherichia coli)

Vogel, H.J.; McLellan, W.L.; Methods Enzymol. 17A, 255-260 (1970)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
gel filtration, enzyme unstable
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
Cu2+
-
Escherichia coli
Fe2+
slight
Escherichia coli
Mn2+
slight
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Ni2+
slight
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
NADP+
-
Escherichia coli
0.6
-
N-acetyl-L-glumate 5-semialdehyde
-
Escherichia coli
3
-
phosphate
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
Escherichia coli
enzyme of arginine biosynthesis
N-acetyl-5-glutamyl phosphate + NADPH
-
Escherichia coli
?
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
Escherichia coli
enzyme of arginine biosynthesis
N-acetyl-5-glutamyl phosphate + NADPH
-
Escherichia coli
r
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
W2D ATCC 25542
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-15C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
in the dehydrogenase reaction, arsenate can substitute for phosphate
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
r
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
enzyme of arginine biosynthesis
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
?
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
enzyme of arginine biosynthesis
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
9
9.5
activity drops sharply below pH 9.0 and above 9.5
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
4C, 5 h
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Escherichia coli
General Stability (protein specific)
General Stability
Organism
gel filtration, enzyme unstable
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
Cu2+
-
Escherichia coli
Fe2+
slight
Escherichia coli
Mn2+
slight
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Ni2+
slight
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.17
-
NADP+
-
Escherichia coli
0.6
-
N-acetyl-L-glumate 5-semialdehyde
-
Escherichia coli
3
-
phosphate
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
Escherichia coli
enzyme of arginine biosynthesis
N-acetyl-5-glutamyl phosphate + NADPH
-
Escherichia coli
?
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
Escherichia coli
enzyme of arginine biosynthesis
N-acetyl-5-glutamyl phosphate + NADPH
-
Escherichia coli
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-15C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
in the dehydrogenase reaction, arsenate can substitute for phosphate
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
r
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
enzyme of arginine biosynthesis
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
?
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
enzyme of arginine biosynthesis
390280
Escherichia coli
N-acetyl-5-glutamyl phosphate + NADPH
-
390280
Escherichia coli
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
9
9.5
activity drops sharply below pH 9.0 and above 9.5
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
4C, 5 h
Escherichia coli
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
690399
Takahara
Continuous spectrophotometric ...
Escherichia coli
Anal. Biochem.
368
138-147
2007
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
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-
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-
1
-
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-
1
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693618
Cherney
Crystal structure of N-acetyl- ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
367
1357-1369
2007
-
-
-
1
-
-
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-
-
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-
-
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3
-
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-
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2
2
1
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-
-
1
-
-
-
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-
-
2
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
654087
Goto
Expression, purification and p ...
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
Acta Crystallogr. Sect. D
59
356-358
2003
-
-
1
1
-
-
-
-
-
-
-
-
-
90
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390290
Abadjieva
A new yeast metabolon involvin ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
42869-42880
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390288
Baetens
Genes and enzymes of the acety ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Microbiology
144
479-492
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
-
-
-
2
-
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-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390289
Parra-Gessert
Processing and function of a p ...
Neurospora crassa
J. Biol. Chem.
273
7972-7980
1998
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
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-
-
1
-
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-
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1
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-
1
-
-
-
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3
-
-
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1730
Wandinger-Ness
Simultaneous purification of t ...
Neurospora crassa
J. Biol. Chem.
261
4820-4827
1986
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
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-
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-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390287
Wandinger-Ness
Acetylglutamate kinase-acetylg ...
Neurospora crassa, Neurospora crassa LA16
J. Biol. Chem.
260
5974-5978
1985
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390286
Jacobs
A cis-dominant regulatory muta ...
Saccharomyces cerevisiae
J. Mol. Biol.
139
691-704
1980
-
-
-
-
-
-
-
-
-
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-
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1
-
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1
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-
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1
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-
1
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-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390285
Minet
Organization and expression of ...
Saccharomyces cerevisiae
Mol. Gen. Genet.
168
299-308
1979
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
1
-
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-
-
-
-
1
-
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390284
Jauniaux
Arginine metabolism in Sacchar ...
Saccharomyces cerevisiae
J. Bacteriol.
133
1096-1107
1978
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
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1
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1
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1
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-
1
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390282
Wipf
-
Compartmentation of arginine b ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae LBG H1323
FEMS Microbiol. Lett.
2
239-242
1977
-
-
-
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
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-
2
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-
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1
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1
-
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2
-
-
2
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390283
Haas
The genetic organization of ar ...
Pseudomonas aeruginosa
Mol. Gen. Genet.
154
7-22
1977
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
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1
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-
1
-
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-
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-
1
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390281
Cybis
Organization and control in th ...
Neurospora crassa
J. Bacteriol.
123
196-202
1975
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
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2
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1
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1
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1
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1
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2
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-
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-
-
-
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-
-
-
-
390280
Vogel
-
N-Acetylglutamic gamma-semiald ...
Escherichia coli
Methods Enzymol.
17A
255-260
1970
-
-
-
-
-
1
8
3
-
-
-
2
-
1
-
-
1
-
-
-
1
1
3
-
-
1
-
-
-
1
1
1
1
-
-
-
-
-
-
1
-
-
1
-
8
-
3
-
-
-
2
-
-
-
1
-
-
1
1
3
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
390279
Glansdorff
Coordination of enzyme synthes ...
Escherichia coli
Biochim. Biophys. Acta
108
308-311
1965
-
-
-
-
-
-
-
-
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1
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1
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2
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1
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1
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1
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
390278
Baich
N-acetyl-gamma-glutamokinase a ...
Escherichia coli, Escherichia coli Wc2
Biochem. Biophys. Res. Commun.
7
491-496
1962
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
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6
-
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-
-
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1
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-
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-
1
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-
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4
-
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-
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6
-
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