General Stability | Organism |
---|---|
gel filtration, enzyme unstable | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
Fe2+ | slight | Escherichia coli | |
Mn2+ | slight | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
Ni2+ | slight | Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
NADP+ | - |
Escherichia coli | |
0.6 | - |
N-acetyl-L-glumate 5-semialdehyde | - |
Escherichia coli | |
3 | - |
phosphate | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | Escherichia coli | enzyme of arginine biosynthesis | N-acetyl-5-glutamyl phosphate + NADPH | - |
? | |
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | Escherichia coli | enzyme of arginine biosynthesis | N-acetyl-5-glutamyl phosphate + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
W2D ATCC 25542 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-15°C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | in the dehydrogenase reaction, arsenate can substitute for phosphate | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
r | |
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | enzyme of arginine biosynthesis | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
? | |
N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | enzyme of arginine biosynthesis | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | activity drops sharply below pH 9.0 and above 9.5 | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | 4°C, 5 h | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |