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Literature summary for 1.2.1.36 extracted from

  • Bchini, R.; Vasiliou, V.; Branlant, G.; Talfournier, F.; Rahuel-Clermont, S.
    Retinoic acid biosynthesis catalyzed by retinal dehydrogenases relies on a rate-limiting conformational transition associated with substrate recognition (2013), Chem. Biol. Interact., 202, 78-84.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Homo sapiens
expressed in Escherichia coli C41(DE3) cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K127A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
N120A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
Y296A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
Y296V the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
retinal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.0029
-
citral wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.0063
-
2,4-decadienal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.0064
-
decanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.0081
-
retinal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
0.0086
-
retinal mutant enzyme K127A, at pH 8.5 and 25°C Homo sapiens
0.0089
-
retinal mutant enzyme Y296A, at pH 8.5 and 25°C Homo sapiens
0.01
-
decanal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
0.011
-
retinal mutant enzyme N120A, at pH 8.5 and 25°C Homo sapiens
0.015
-
retinal mutant enzyme Y296V, at pH 8.5 and 25°C Homo sapiens
0.023
-
hexanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
retinal + NAD+ + H2O Homo sapiens
-
retinoate + NADH + H+
-
?
retinal + NAD+ + H2O Rattus norvegicus
-
retinoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-Sepharose column chromatography and Q-Sepharose column chromatography Homo sapiens
nickel-Sepharose column chromatography and Q-Sepharose column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-decadienal + NAD+ + H2O
-
Homo sapiens 2,4-decadienoate + NADH + H+
-
?
2,4-decadienal + NAD+ + H2O
-
Rattus norvegicus 2,4-decadienoate + NADH + H+
-
?
citral + NAD+ + H2O
-
Homo sapiens (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+
-
?
citral + NAD+ + H2O
-
Rattus norvegicus (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+
-
?
decanal + NAD+ + H2O
-
Homo sapiens decanoate + NADH + H+
-
?
decanal + NAD+ + H2O
-
Rattus norvegicus decanoate + NADH + H+
-
?
hexanal + NAD+ + H2O
-
Homo sapiens hexanoate + NADH + H+
-
?
hexanal + NAD+ + H2O
-
Rattus norvegicus hexanoate + NADH + H+
-
?
retinal + NAD+ + H2O
-
Homo sapiens retinoate + NADH + H+
-
?
retinal + NAD+ + H2O
-
Rattus norvegicus retinoate + NADH + H+
-
?
retinol + NAD+ + H2O
-
Homo sapiens ? + NADH + H+
-
?
retinol + NAD+ + H2O
-
Rattus norvegicus ? + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
ALDH1A1
-
Homo sapiens
Aldh1a2
-
Rattus norvegicus
NAD-dependent retinal dehydrogenase
-
Rattus norvegicus
NAD-dependent retinal dehydrogenases
-
Homo sapiens
RalDH1
-
Homo sapiens
RALDH2
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
retinal mutant enzyme Y296V, at pH 8.5 and 25°C Homo sapiens
0.017
-
retinal mutant enzyme Y296A, at pH 8.5 and 25°C Homo sapiens
0.03
-
retinal mutant enzyme K127A, at pH 8.5 and 25°C Homo sapiens
0.03
-
retinal mutant enzyme N120A, at pH 8.5 and 25°C Homo sapiens
0.07
-
retinal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
0.1
-
retinal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.1
-
citral wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
0.15
-
2,4-decadienal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
2
-
hexanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
2.2
-
decanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NAD+
-
Rattus norvegicus