Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
glutathione | - |
Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | RDH13 is significantly different from related RDH11, RDH12 and RDH14 in that it is targeted to the mitochondria, but at the same time, RDH13 is very similar to the members of the RDH11-14 cluster of short-chain dehydrogenases/reductases in terms of its substrate and cofactor preferences. It may function to protect mitochondria against oxidative stress associated with the highly reactive retinaldehyde produced from dietary beta-carotene | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloned into the pET28a vector. Expressed in Sf9 cells as a fusion with the C-terminal His6 tag | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | if dithiothreitol is omitted from the elution buffer during RDH13-His6 purification, the purified enzyme has a very low activity, but can be reactivated by the addition of dithiothreitol. Becomes partially inactivated after 20 min of incubation at 37°C. Not inhibited at 0.05 mM by nonanal, 6-cis-nonenal and 2-trans-nonenal, glyceraldehyde and acetoacetyl-coenzyme A, taurocholic acid, 25-hydroxycholesterol and 25-nor-5-cholesten-3-ol-25b-one | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
NADPH | - |
Homo sapiens | |
0.003 | - |
all-trans-retinol | - |
Homo sapiens | |
0.0032 | - |
all-trans-retinaldehyde | - |
Homo sapiens | |
6 | - |
NADH | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | outer side | Homo sapiens | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
immunoblotting | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RDH13-His6 purified by Ni2+ affinity chromatography, to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | abundant | Homo sapiens | - |
kidney | abundant | Homo sapiens | - |
LNCaP cell | expresses endogenous RDH13 at high levels | Homo sapiens | - |
lung | abundant | Homo sapiens | - |
ovary | - |
Homo sapiens | - |
prostate | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
- |
Homo sapiens |
0.13 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
all-trans-retinaldehyde + NAD(P)H | - |
Homo sapiens | NAD(P)+ + H2O + all-trans-retinoate | - |
? | |
all-trans-retinol + NAD(P)H | - |
Homo sapiens | NAD(P)+ + H2O + all-trans-retinaldehyde | - |
? | |
additional information | no significant conversion of 17beta-, 3alpha- and 11beta-hydroxysteroids, and corresponding ketosteroids | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RDH13 | - |
Homo sapiens |
retinol dehydrogenase 13 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens | |
NADPH | prefers NADPH to NADH as a cofactor | Homo sapiens |