Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) as His-tag fusion protein | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 0.22 mM | Xenopus laevis | |
Mg2+ | 50% inhibition at 0.035 mM | Xenopus laevis | |
Mn2+ | 50% inhibition at 0.024 mM | Xenopus laevis | |
Zn2+ | 50% inhibition at 0.01 mM | Xenopus laevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
retinal | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.0044 | - |
retinal | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.5 mM Ca2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.0046 | - |
retinal | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.04 mM Mn2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.006 | - |
retinal | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Mg2+, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.0064 | - |
retinal | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Zn2+ 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.019 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.024 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 Mn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.027 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Ca2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.028 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.055 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
1.4 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Ca2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
1.8 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
2 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 mM Mn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
2.1 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
3.4 | - |
acetaldehyde | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE, recombinant protein | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity chromatography | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Xenopus laevis | acetate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | - |
Xenopus laevis | retinoic acid + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase 1A1 | - |
Xenopus laevis |
ALDH class 1 | - |
Xenopus laevis |
ALDH1 | - |
Xenopus laevis |
ALDH1A1 | - |
Xenopus laevis |
RalDH1 | - |
Xenopus laevis |
retinaldehyde dehydrogenase | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Xenopus laevis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Zn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.011 | - |
Zn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
0.015 | - |
Mn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.036 | - |
Zn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.04 | - |
Mn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
0.052 | - |
Mn2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.11 | - |
Mg2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.17 | - |
Mg2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
0.27 | - |
Ca2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+ | Xenopus laevis | |
0.3 | - |
Ca2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+ | Xenopus laevis | |
0.349 | - |
Mg2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis | |
0.479 | - |
Ca2+ | 50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+ | Xenopus laevis |