Cloned (Comment) | Organism |
---|---|
Lys5 and Lys2 cloned from Saccharomyces cerevisiae DNA, overexpressed in Escherichia coli, and purified, Lys5/Lys2 pair is a two component system in which Lys5 covalently primes Lys2, allowing alpha-aminoadipate reductase activity by holo-Lys2 with catalytic cycles of autoaminoacylation and reductive clevage | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
NADPH | of Lys2 | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
Lys5 from induced E. coli cells | Saccharomyces cerevisiae |
155000 | - |
full-length Lys2 from induced E. coli cells | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alpha-aminoadipate + NAD(P)H | Saccharomyces cerevisiae | - |
L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | Lys5 demonstrates phosphopantetheinyl transferase activity toward the 14-kDa Lys2 peptidyl carrier protein fragment, with a Km of 0.001 mM and a turnover number of 3/min | Saccharomyces cerevisiae |
side-chain modification | Lys5 is a specific posttranslational modification catalyst, using coenzyme A as a cosubstrate to phosphopantetheinylate Ser880 of the Lys2 activating it for catalysis | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-aminoadipic semialdehyde + NAD+ + H2O | - |
Saccharomyces cerevisiae | alpha-aminoadipic acid + NADH + H+ | - |
? | |
L-2-aminoadipate + NADPH | - |
Saccharomyces cerevisiae | L-2-aminoadipate 6-semialdehyde + NADP+ + H2O | - |
? | |
L-alpha-aminoadipate + NAD(P)H | - |
Saccharomyces cerevisiae | L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.2 | - |
alpha-aminoadipate semialdehyde | production from holo-lys2 | Saccharomyces cerevisiae |