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Literature summary for 1.2.1.30 extracted from
- Reduction of carboxylic acids by Nocardia aldehyde oxidoreductase requires a phosphopantetheinylated enzyme (2007), J. Biol. Chem., 282, 478-485.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphopantetheine transferase | required for activating the enzyme. Recombinant car is an apoenzyme that requires phosphopantetheinylation for conversion to a fully active holoenzyme | Nocardia iowensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant car expressed in Escherichia coli BL21-CodonPlus(DE3)-RP/pHAT305 and in Escherichia coli BL21-CodonPlus(DE3)-RP/pPV1.184 cells | Nocardia iowensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S689A | completely inactive | Nocardia iowensis |
| S691A | has 39% of the activity of recombinant car | Nocardia iowensis |
| S694A | has 50% of the activity of recombinant car | Nocardia iowensis |
| S696A | has 76% of the activity of recombinant car | Nocardia iowensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Nocardia iowensis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardia iowensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Nocardia iowensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.3 | - |
recombinant car incubated with CoA | Nocardia iowensis |
| 0.5 | - |
Nocardia cell free extract incubated with recombinant car | Nocardia iowensis |
| 1.5 | - |
recombinant car incubated with CoA and Escherichia coli MV1190/pUC8-sfp cell free extract | Nocardia iowensis |
| 1.6 | - |
Nocardia cell free extract incubated with recombinant car and CoA. Recombinant car incubated with CoA and Npt | Nocardia iowensis |
| 2.1 | - |
car purified from BL21-CodonPlus(DE3)-RP/pPV1.184 (car and sfp) | Nocardia iowensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoate + NADPH + ATP | - |
Nocardia iowensis | benzaldehyde + NADP+ + AMP + phosphate | - |
? | |
| vanillic acid + NADPH + H+ + ATP | with Escherichia coli BL21-CodonPlus(DE3)-RP/pPV2.83, in which recombinant Npt is expressed along with recombinant car, vanillic acid is reduced to vanillin and vanillyl alcohol, with vanillin (80%) as the major product. Escherichia coli BL21-CodonPlus(DE3)-RP/pHAT305 (expressing only recombinant Car) reduce only 50% of the vanillic acid starting material, with vanillyl alcohol being the major metabolite. With Escherichia coli BL21-CodonPlus(DE3)-RP/pPV2.83, in which recombinant car is presumed to be in the fully active, phosphopantetheinylated holo form, the rate of reduction of vanillic acid is much faster than that of vanillin to vanillyl alcohol by endogenous Escherichia coli aldehyde dehydrogenase | Nocardia iowensis | vanillin + vanillyl alcohol + NADP+ + AMP + diphosphate | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Nocardia iowensis | |
| NADPH | - |
Nocardia iowensis | |
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Release 2023.1 (January 2023)
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