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Literature summary for 1.2.1.30 extracted from

  • Gross, G.G.; Zenk, M.H.
    Reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 1. Purification and properties of aryl-aldehyde: NADP-oxidoreductase from Neurospora crassa (1969), Eur. J. Biochem., 8, 413-419.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.152
-
 ATP
-
 Neurospora crassa
0.167
-
 salicylate
-
 Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aromatic acids + NADPH + H+ + ATP Neurospora crassa
-
 aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by precipitation of nucleic acids with protamine sulfate, ammonium sulphate fractionation, adsorption on calcium-phosphate gel and chromatography on TEAE-cellulose and on hydroxylapatite Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.864
-
-
 Neurospora crassa

Storage Stability

Storage Stability Organism
0°C, 2 weeks Neurospora crassa
0°C, plus reagents with SH-groups, 3 weeks Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aromatic acids + NADPH + H+ + ATP
-
 Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ?
aromatic acids + NADPH + H+ + ATP
-
 Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
 ir
benzoic acid + NADPH + H+ + ATP rate relative to salycilate 286% Neurospora crassa benzaldehyde + NADP+ + AMP + diphosphate
-
 ?
cinnamic acid + NADPH + ATP
-
 Neurospora crassa 3-phenyl-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
m-coumaric acid + NADPH + ATP
-
 Neurospora crassa 3-(3-hydroxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
m-hydroxybenzoic acid + NADPH + ATP
-
 Neurospora crassa m-hydroxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
o-coumaric acid + NADPH + ATP
-
 Neurospora crassa 3-(2-hydroxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
p-anisic acid + NADPH + ATP
-
 Neurospora crassa p-methoxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
p-coumaric acid + NADPH + ATP
-
 Neurospora crassa 3-(4-hydroxphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
 ?
p-hydroxybenzoic acid + NADPH + ATP
-
 Neurospora crassa p-hydroxybenzaldehyde + NADP+ + AMP + H2O
-
 ?
salicylic acid + NADPH + ATP
-
 Neurospora crassa salicyl aldehyde + NADP+ + AMP + H2O
-
 ?
vanillic acid + NADPH + H+ + ATP
-
 Neurospora crassa 4-hydroxy-3-methoxybenzaldehyde + NADP+ + AMP + diphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 36 salicylate + ATP + NADPH Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 8.1 salicylate + ATP + NADPH Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
ATP completely dependent on Neurospora crassa
NADPH completely dependent on Neurospora crassa