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Literature summary for 1.2.1.30 extracted from
- Formation and reduction of intermediate acyladenylate by aryl-aldehyde. NADP oxidoreductase from Neurospora crassa (1972), Eur. J. Biochem., 31, 585-592.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | stimulation of the reduction of the intermediate benzoyladenosine 5'-monophosphate to benzaldehyde | Neurospora crassa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | inhibition of the exchange reaction of diphosphate and ATP | Neurospora crassa | |
| hydroxylamine | inhibition of the exchange reaction of diphosphate and ATP | Neurospora crassa | |
| iodoacetamide | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
| iodoacetate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
| N-ethylmaleimide | inhibition of the reduction of benzoyladenosine 5'-monophosphate | Neurospora crassa | |
| NADPH | inhibition of the exchange reaction of diphosphate and ATP, inhibitory effect increases when dithiothreitol is added | Neurospora crassa | |
| o-Iodosobenzoate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
| p-chloromercuribenzoate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
| p-diazobenzenesulfonate | inhibition of the reduction of benzoyladenosine 5'-monophosphate, even in the presence of dithiothreitol | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
benzoate | - |
Neurospora crassa | |
| 0.13 | - |
NADPH | reduction of the intermediate benzoyladenylate to benzaldehyde | Neurospora crassa | |
| 0.2 | - |
benzoyladenosine 5'-monophosphate | - |
Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aromatic acids + NADPH + H+ + ATP | Neurospora crassa | - |
aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| pH 7.6, 50% glycerol, 1 mM EDTA, 2.5 mM mercaptoethanol, 6 months | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aromatic acids + NADPH + H+ + ATP | - |
Neurospora crassa | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
? | |
| aromatic acids + NADPH + H+ + ATP | - |
Neurospora crassa | aromatic aldehydes + NADP+ + AMP + diphosphate + H2O | - |
r | |
| benzaldehyde + NADP+ + benzoyladenosine 5'-monophosphate + phosphate | - |
Neurospora crassa | benzoate + NADPH + ATP | - |
r | |
| benzoic acid + NADPH + H+ + ATP | the reaction proceeds via benzoyladenosine 5'-monophosphate which is further reduced by NADPH to benzaldehyde | Neurospora crassa | benzaldehyde + NADP+ + AMP + diphosphate | - |
? | |
| cinnamic acid + NADPH + ATP | - |
Neurospora crassa | 3-phenyl-2-propen-1-al + NADP+ + AMP + H2O | - |
? | |
| m-hydroxybenzoic acid + NADPH + ATP | - |
Neurospora crassa | m-hydroxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
| p-anisic acid + NADPH + ATP | - |
Neurospora crassa | p-methoxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
| p-coumaric acid + NADPH + ATP | - |
Neurospora crassa | 3-(4-hydroxphenyl)-2-propen-1-al + NADP+ + AMP + H2O | - |
? | |
| p-hydroxybenzoic acid + NADPH + ATP | - |
Neurospora crassa | p-hydroxybenzaldehyde + NADP+ + AMP + H2O | - |
? | |
| salicylic acid + NADPH + ATP | rate relative to benzoate 9% | Neurospora crassa | salicyl aldehyde + NADP+ + AMP + H2O | - |
? | |
| vanillic acid + NADPH + H+ + ATP | - |
Neurospora crassa | 4-hydroxy-3-methoxybenzaldehyde + NADP+ + AMP + diphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
for the reduction of benzoyladenosine 5'-monophosphate | Neurospora crassa |
| 8.4 | 9.2 | for the exchange reaction of diphosphate and ATP | Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
not active below, benzoate + ATP | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Neurospora crassa | |
| NADPH | - |
Neurospora crassa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
AMP | - |
Neurospora crassa | |
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