Cloned (Comment) | Organism |
---|---|
gene ALDH16, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus | Loktanella sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type enzyme LsALDH16 and enzyme mutant C295A free or in complex with NAD+ or NADH or 4-nitrophenylacetate, hanging drop vapor diffusion, mixing of 6 mg/ml in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 2.5% glycerol, and 0.5 mM tris(2-carboxyethyl)phosphine, with reservoir solution and microseeding solution in a volume ratio of 1:1:0.2, where the reservoir solution comprisess 20% w/v PEG 3350, 200 mM ammonium sulfate, and 100 mM Bis-Tris, pH 5.5, and the microseed solution is made from crushed crystals diluted 1:100 in reservoir, at room temperature, crystals of mutant C295A complexed with 4-nitrophenylacetate are prepared by soaking the apo crystals with the reservoir solution supplemented with 10 mM ligand for about 30 minutes, X-ray diffraction structure determination and analysis at 1.49-2.30 A resolution | Loktanella sp. |
Protein Variants | Comment | Organism |
---|---|---|
C295A | site-directed mutagenesis, inactive mutant | Loktanella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenylacetate | substrate inhibition of esterase activity | Loktanella sp. | |
N,N-diethylaminobenzaldehyde | DEAB, complete inhibition | Loktanella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics and modeling | Loktanella sp. | |
0.0213 | - |
hexanal | pH 8.0, 22°C, recombinant wild-type enzyme | Loktanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IZ83 | - |
- |
Loktanella sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Codon Plus by nickel affinity chromatography, anion exchange chromatography, and gel filtration | Loktanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexanal + NAD+ + H2O | - |
Loktanella sp. | hexanoate + NADH + H+ | - |
? | |
additional information | enzyme LsALDH16 also exhibits esterase activity with 4-nitrophenylacetate (pNPA) as substrate in the absence of NAD+, kinetics, overview | Loktanella sp. | ? | - |
- |
|
additional information | no activity with hexanal, propanal, nonanal, acetaldehyde and aminoadipate semialdehyde | Homo sapiens | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80792, mass spectrometry | Loktanella sp. |
dimer | 2 * 85395, mass spectrometry | Homo sapiens |
More | the fold of ALDH16 comprises three domains: NAD+-binding, catalytic, and C-terminal. The latter is unique to ALDH16 and features a Rossmann fold connected to a protruding beta-flap. The tertiary structural interactions of the C-terminal domain mimic the quaternary structural interactions of the classic ALDH superfamily dimer, a phenomenon that is called trans-hierarchical structural similarity. ALDH16 forms a unique dimer in solution, which mimics the classic ALDH superfamily dimer-of-dimers tetramer | Loktanella sp. |
More | the fold of ALDH16 comprises three domains: NAD+-binding, catalytic, and C-terminal. The latter is unique to ALDH16 and features a Rossmann fold connected to a protruding beta-flap. The tertiary structural interactions of the C-terminal domain mimic the quaternary structural interactions of the classic ALDH superfamily dimer, a phenomenon that is called trans-hierarchical structural similarity. ALDH16 forms a unique dimer in solution, which mimics the classic ALDH superfamily dimer-of-dimers tetramer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase 16 | - |
Loktanella sp. |
aldehyde dehydrogenase 16 | - |
Homo sapiens |
ALDH16 | - |
Loktanella sp. |
ALDH16 | - |
Homo sapiens |
HsALDH16A1 | - |
Homo sapiens |
LsALDH16 | - |
Loktanella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Loktanella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
hexanal | pH 8.0, 22°C, recombinant wild-type enzyme | Loktanella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Loktanella sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | enzyme binding analysis, overview | Loktanella sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
4-nitrophenylacetate | pH and temperature not specified in the publication | Loktanella sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the aldehyde dehydrogenase (ALDH) superfamily. Enzyme ALDH16 has an extra C-terminal domain of unknown function and shows absence of the essential catalytic cysteine residue in certain non-bacterial ALDH16 sequences | Homo sapiens |
evolution | the enzyme belongs to the aldehyde dehydrogenase (ALDH) superfamily. Enzyme ALDH16 has an extra C-terminal domain of unknown function and shows absence of the essential catalytic cysteine residue in certain non-bacterial ALDH16 sequences. The Loktanella ALDH16 structure may be considered to be the archetype of the ALDH16 family | Loktanella sp. |
additional information | active site structure modeling of LsALDH16 from 1.65 A resolution crystal structure with the catalytic loop in complex with NAD+. Structure comparison of the human and the Loktanella sp. ALDH16 enzymes, overview | Loktanella sp. |
additional information | structure comparison of the human and the Loktanella sp. ALDH16 enzymes, overview | Homo sapiens |
physiological function | human ALDH16A1 apparently lacks measurable aldehyde oxidation activity, suggesting it is a pseudoenzyme, consistent with the absence of the catalytic Cys in its sequence | Homo sapiens |
physiological function | Loktanella ALDH16 is a bona fide enzyme, exhibiting NAD+-binding | Loktanella sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
154.93 | - |
hexanal | pH 8.0, 22°C, recombinant wild-type enzyme | Loktanella sp. |