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Literature summary for 1.2.1.3 extracted from
- Spectrophotometric assay for sensitive detection of glycerol dehydratase activity using aldehyde dehydrogenase (2017), J. Biosci. Bioeng., 123, 528-533 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | spectrophotometric assay for the sensitive measurement of the glycerol dehydratase activity with a sub-nanomolar limit of detection. The assay method employs aldehyde dehydrogenase as a reporter enzyme, so the readout of the glycerol dehydratase activity is recorded at 340 nm as an increase in UV absorbance which results from NADH generation accompanied by oxidation of 3-hydroxypropionaldehyde to 3-hydroxypropionic acid. The glycerol dehydratase assay is performed under the reaction conditions where the aldehyde dehydrogenase activity overwhelms the GDHt activity (i.e., 50fold higher activity of aldehyde dehydrogenase relative to glycerol dehydratase activity), affording sensitive detection of glycerol dehydratase with 360 pM limit of detection | Klebsiella pneumoniae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the dhaB gene in Escherichia coli BL21(DE3) | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
| Klebsiella pneumoniae DSM 2026 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropanal + NAD+ + H2O | - |
Klebsiella pneumoniae | 3-hydroxypropionate + NADH + H+ | - |
? |  |
| 3-hydroxypropanal + NAD+ + H2O | - |
Klebsiella pneumoniae DSM 2026 | 3-hydroxypropionate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae | benzoate + NADH + H+ | - |
? | |
| benzaldehyde + NAD+ + H2O | - |
Klebsiella pneumoniae DSM 2026 | benzoate + NADH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Klebsiella pneumoniae |
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