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Literature summary for 1.2.1.3 extracted from

  • Belmont-Diaz, J.A.; Yoval-Sanchez, B.; Calleja-Castaneda, L.F.; Pardo Vazquez, J.P.; Rodriguez-Zavala, J.S.
    Alda-1 modulates the kinetic properties of mitochondrial aldehyde dehydrogenase (ALDH2) (2016), FEBS J., 283, 3637-3650.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Alda-1 acts as activator at high concentrations of aldehyde Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Alda-1 acts as inhibitor at low concentrations of aldehyde Homo sapiens
NADH
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
propanal in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens
0.006
-
propanal in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens
0.03
-
NAD+ in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens
0.073
-
NAD+ in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanal + NAD+ + H2O
-
Homo sapiens propanoate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
ALDH2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
NAD+ in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens
1.9
-
propanal in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens
3.05
-
NAD+ in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens
3.05
-
propanal in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.053
-
NADH in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens
0.133
-
NADH in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.027
-
NAD+ in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens
0.117
-
NAD+ in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens
0.517
-
propanal in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C Homo sapiens
3.13
-
propanal in the absence of Alda-1, at pH 7.4 and 25°C Homo sapiens