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Literature summary for 1.2.1.3 extracted from
- Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021 (2011), J. Biol. Chem., 286, 22283-22290.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required | Sinorhizobium meliloti |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Sinorhizobium meliloti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
phosphonoacetaldehyde | at pH 7.5 and 30°C | Sinorhizobium meliloti | |
| 1 | - |
NAD+ | at pH 7.5 and 30°C | Sinorhizobium meliloti | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55076 | - |
x * 55076, His-tagged enzyme, calculated from amino acid sequence | Sinorhizobium meliloti |
| 55100 | - |
x * 55100, His-tagged enzyme, SDS-PAGE | Sinorhizobium meliloti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphonoacetaldehyde + NAD+ | Sinorhizobium meliloti | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography, and gel filtration | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphonoacetaldehyde + NAD+ | - |
Sinorhizobium meliloti | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55076, His-tagged enzyme, calculated from amino acid sequence | Sinorhizobium meliloti |
| ? | x * 55100, His-tagged enzyme, SDS-PAGE | Sinorhizobium meliloti |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phnY | - |
Sinorhizobium meliloti |
| phosphonoacetaldehyde dehydrogenase | - |
Sinorhizobium meliloti |
| PnAA dehydrogenase | - |
Sinorhizobium meliloti |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sinorhizobium meliloti | |
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