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Literature summary for 1.2.1.3 extracted from

  • Stagos, D.; Chen, Y.; Brocker, C.; Donald, E.; Jackson, B.C.; Orlicky, D.J.; Thompson, D.C.; Vasiliou, V.
    Aldehyde dehydrogenase 1B1: molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme (2010), Drug Metab. Dispos., 38, 1679-1687.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in in Sf9 insect cells and in Mus musculus (highest human ALDH1B1 mRNA expression is detected in liver and part of the small intestine, moderate levels in the testis, distal colon, lung, heart, and duodenum and the lowest levels are found in the kidney and stomach) Homo sapiens

Protein Variants

Protein Variants Comment Organism
E487K the mutation results in poor binding affinity to cofactor NAD+ and about 90% loss of enzyme activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Disulfiram at higher concentrations of propionaldehyde (0.5 and 1 mM), ALDH1B1 activity is inhibited by disulfiram, with the extent of inhibition being about 40% at 0.01 mM disulfiram Homo sapiens
additional information at the lowest concentration of substrate tested (propionaldehyde 0.1 mM), ALDH1B1 is not affected by disulfiram Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 hexanal at 25°C, pH not specified in the publication Homo sapiens
0.0008
-
 n-nonanal at 25°C, pH not specified in the publication Homo sapiens
0.0036
-
 NAD+ at 25°C, pH not specified in the publication Homo sapiens
0.014
-
 propionaldehyde at 25°C, pH not specified in the publication Homo sapiens
0.05
-
 benzaldehyde at 25°C, pH not specified in the publication Homo sapiens
0.055
-
 acetaldehyde at 25°C, pH not specified in the publication Homo sapiens
0.466
-
 malondialdehyde at 25°C, pH not specified in the publication Homo sapiens
3.383
-
 4-hydroxy-2-nonenal at 25°C, pH not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
5'-AMP-Sepharose 4B affinity column chromatography, gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-nonenal + NAD+ + H2O low affinity for substrate binding and poor catalytic efficiency Homo sapiens 4-hydroxy-2-nonenoate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
hexanal + NAD+ + H2O
-
 Homo sapiens hexanoate + NADH + H+
-
 ?
malondialdehyde + NAD+ + H2O low affinity for substrate binding and poor catalytic efficiency Homo sapiens ?
-
 ?
additional information no activity with NADP+ and unsaturated aldehydes like trans-2-hexenal, trans-2-nonenal, and trans-2 dodecenal Homo sapiens ?
-
 ?
additional information the enzyme shows esterase activity with 4-nitrophenyl acetate Homo sapiens ?
-
 ?
n-nonanal + NAD+ + H2O
-
 Homo sapiens n-nonanoate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase 1B1 isoform Homo sapiens
Aldh1b1 isoform Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ isoform ALDH1B1 has an exclusive preference for NAD+ as the cofactor, no catalytic activity occurs when NADP+ is used Homo sapiens