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Literature summary for 1.2.1.3 extracted from
- Purification, characterization and cloning of aldehyde dehydrogenase from Rhodococcus erythropolis UPV-1 (2007), Appl. Microbiol. Biotechnol., 73, 1073-1086.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| when expressed in Escherichia coli M15 most of the protein is localised in inclusion bodies, not used for the enzyme assays | Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | 7% inhibition at 1 mM | Rhodococcus erythropolis |  |
| EDTA | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
| iodacetamide | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
| KCN | 10-20% inhibition at 1 mM | Rhodococcus erythropolis | |
| methanol | 17% inhibition at 1 mM | Rhodococcus erythropolis | |
| Ni2+ | 52% inhibition at 1 mM | Rhodococcus erythropolis | |
| p-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Rhodococcus erythropolis | |
| Zn2+ | 73% inhibition at 1 mM | Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000085 | - |
octanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
| 0.0018 | - |
hexanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
| 0.0038 | - |
Butanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
| 0.0091 | - |
propanal | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
| 0.09 | - |
NAD+ | 0,05 mM propanal, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
| 0.333 | - |
formaldehyde | 1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C | Rhodococcus erythropolis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 23% activation at 1 mM | Rhodococcus erythropolis | |
| NH4+ | 13% activation at 1 mM | Rhodococcus erythropolis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16200 | - |
non-denaturing PAGE | Rhodococcus erythropolis |
| 54000 | - |
3 * 54000, non-denaturing PAGE | Rhodococcus erythropolis |
| 54680 | - |
MALDI-TOF MS | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | Q4F895 | - |
- |
| Rhodococcus erythropolis UPV-1 | Q4F895 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two chromatography steps to homogeneity | Rhodococcus erythropolis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Rhodococcus erythropolis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.5 | - |
- |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butanal + NAD+ + H2O | - |
Rhodococcus erythropolis | butyrate + NADH + H+ | - |
? | |
| butanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | butyrate + NADH + H+ | - |
? | |
| formaldehyde + NAD+ + H2O | - |
Rhodococcus erythropolis | formate + NADH | - |
? | |
| formaldehyde + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | formate + NADH | - |
? | |
| hexanal + NAD+ + H2O | - |
Rhodococcus erythropolis | hexanoate + NADH + H+ | - |
? | |
| hexanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | hexanoate + NADH + H+ | - |
? | |
| octanal + NAD+ + H2O | - |
Rhodococcus erythropolis | octanoate + NADH | - |
? | |
| octanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | octanoate + NADH | - |
? | |
| propanal + NAD+ + H2O | - |
Rhodococcus erythropolis | propanoate + NADH + H+ | - |
? | |
| propanal + NAD+ + H2O | - |
Rhodococcus erythropolis UPV-1 | propanoate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 54000, non-denaturing PAGE | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aldehyde dehydrogenase | - |
Rhodococcus erythropolis |
| ALDH | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47.5 | - |
with n-propanal as substrate | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
fully stable after 10 min | Rhodococcus erythropolis |
| 40 | - |
75% activity after 10 min in presence or absence of substrates | Rhodococcus erythropolis |
| 40 | - |
fully active after 10 min when incubated with NAD+ | Rhodococcus erythropolis |
| 60 | - |
completely inactive after 10 min | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
with n-propanal as substrate | Rhodococcus erythropolis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 60% activity after 16 h at 4°C | Rhodococcus erythropolis |
| 9.5 | - |
10% activity when stored at a pH above 9.5 | Rhodococcus erythropolis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | totally inactive with NADP+ | Rhodococcus erythropolis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodococcus erythropolis | two-dimensional electrophoresis | - |
5.3 |
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