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Literature summary for 1.2.1.3 extracted from

  • Fong, W.P.; Cheng, C.H.K.; Tang, W.K.
    Purification and characterization of two distinct aldehyde oxidizing enzymes from the liver of black seabream (2003), Chem. -Biol. Interact., 143-144, 159-167.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
propanal pH 9.5 Acanthopagrus schlegelii
0.007
-
octanal pH 9.5 Acanthopagrus schlegelii
0.009
-
acetaldehyde pH 9.5 Acanthopagrus schlegelii
0.4
-
NAD+ pH 9.5, reaction with acetaldehyde Acanthopagrus schlegelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, SDS-PAGE Acanthopagrus schlegelii

Organism

Organism UniProt Comment Textmining
Acanthopagrus schlegelii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acanthopagrus schlegelii

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Acanthopagrus schlegelii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
-
Acanthopagrus schlegelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Acanthopagrus schlegelii acetate + NADH + H+
-
?
additional information no activity with benzaldehyde Acanthopagrus schlegelii ?
-
?
octanal + NAD+ + H2O
-
Acanthopagrus schlegelii octanoate + NADH
-
?
propanal + NAD+ + H2O
-
Acanthopagrus schlegelii propanoate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Acanthopagrus schlegelii

Synonyms

Synonyms Comment Organism
ALDH-2
-
Acanthopagrus schlegelii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
pH 7.5, 10 min, stable in presence of 2 mM NAD+ Acanthopagrus schlegelii
30
-
pH 7.5, 10 min, about 25% loss of activity in presence of 2 mM NAD+ Acanthopagrus schlegelii
40
-
pH 7.5, 10 min, about 75% loss of activity Acanthopagrus schlegelii
50
-
pH 7.5, 10 min, complete loss of activity Acanthopagrus schlegelii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Acanthopagrus schlegelii

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 40% of maximal activity, pH 10.0: about 60% of maximal activity, reaction with acetaldehyde Acanthopagrus schlegelii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 10 min, stable in presence of 2 mM NAD+ Acanthopagrus schlegelii