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Literature summary for 1.2.1.3 extracted from
- Human liver aldehyde dehydrogenases: new method of purification of the major mitochondrial and cytosolic enzymes and re-evaluation of their kinetic properties (1994), Biochim. Biophys. Acta, 1205, 301-307.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
acetaldehyde | enzyme ALDH-1, pH 7.5 | Homo sapiens |  |
| 0.2 | - |
acetaldehyde | enzyme ALDH-2, pH 9.5 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | enzyme ALDH-1 | Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, enzyme form ALDH-2, SDS-PAGE | Homo sapiens |
| 58000 | - |
x * 58000, enzyme form ALDH-1, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.137 | - |
- |
Homo sapiens |
| 5.5 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, pH 6.0, 30 mM potassium phosphate, 1 mM EDTA, 1 nN dithiothreitol, 2 mM benzamidine, 70 mM MES, after half a year of storage with repeated freezing and thawing the activity of both ALDH-1 and ALDH-2 decrease 2fold to 3fold | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, enzyme form ALDH-2, SDS-PAGE | Homo sapiens |
| ? | x * 58000, enzyme form ALDH-1, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 13.2 | - |
acetaldehyde | enzyme form ALDH-1, pH 9.5 | Homo sapiens | |
| 19.7 | - |
acetaldehyde | enzyme form ALDH-2, pH 9.5 | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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