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Literature summary for 1.2.1.3 extracted from

  • Wei, B.; Ni, L.; Hurley, T.D.; Weiner, H.
    Cooperativity in Nicotinamide Adenine Dinucleotide Binding Induced by Mutations of Arginine 475 Located at the Subunit Interface in the Human Liver Mitochondrial Class 2 Aldehyde Dehydrogenase (2000), Biochemistry, 39, 5295-5302.
    View publication on PubMed
Search for more literature for 1.2.1.3

Protein Variants

Protein Variants Comment Organism
R264E turnover-number is about 6% of that of the native enzyme, Km-value is about 20fold higher than the Km-value of the wild-type enzyme Homo sapiens
R264Q turnover-number is about 50% of that of the native enzyme, Km-value is about 1.6fold higher than the Km-value of the wild-type enzyme Homo sapiens
R475E turnover-number is about 9% of that of the native enzyme, Km-value is about 35fold higher than the Km-value of the wild-type enzyme Homo sapiens
R475E/R264E turnover-number is about 2% of that of the native enzyme, Km-value is about 430fold higher than the Km-value of the wild-type enzyme Homo sapiens
R475Q positive cooperativity in NAD+ binding, Km-value increases 23fold, mutant enzyme is thermally less stable than the native enzyme, the presence of NAD+ restores nativelike stability to the mutant Homo sapiens
R475Q/R264Q Km-value is about 35fold higher than the KM-value of the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of the 475 and 264 arginine mutants of Oriental variant E847K Homo sapiens
0.037
-
 NAD+ native enzyme Homo sapiens
0.06
-
 NAD+ mutant enzyme R264Q Homo sapiens
0.74
-
 NAD+ mutant enzyme R274E Homo sapiens
0.85
-
 NAD+ mutant enzyme R475Q Homo sapiens
1.3
-
 NAD+ mutant enzyme R475Q/R264Q and R475E Homo sapiens
16
-
 NAD+ mutant enzyme R475E/R264E Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion enzyme ALDH-2 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 min, mutant enzyme R475Q loses 50% of its activity Homo sapiens
50
-
 2 min, complete loss of activity of mutant enzyme R475Q, native enzyme loses 10% of itst activity Homo sapiens
55
-
 1 min, 50% loss of activity of native enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover numbers of the 475 and 264 arginine mutants of Oriental variant E847K Homo sapiens
0.0667
-
 NAD+ mutant enzyme R475E/R264E Homo sapiens
0.2
-
 NAD+ mutant enzyme R264E Homo sapiens
0.3
-
 NAD+ mutant enzyme R475E Homo sapiens
2
-
 NAD+ mutant enzyme R475Q Homo sapiens
2.08
-
 NAD+ mutant enzyme R264Q Homo sapiens
3.5
-
 NAD+ native enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens