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Literature summary for 1.2.1.28 extracted from

  • Saini, S.S.; Teotia, D.; Gaid, M.; Thakur, A.; Beerhues, L.; Sircar, D.
    Benzaldehyde dehydrogenase-driven phytoalexin biosynthesis in elicitor-treated Pyrus pyrifolia cell cultures (2017), J. Plant Physiol., 215, 154-162 .
    View publication on PubMed
Search for more literature for 1.2.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00052
-
 benzaldehyde pH 9.5, 40°C Pyrus pyrifolia
0.064
-
 NAD+ pH 9.5, 40°C Pyrus pyrifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Pyrus pyrifolia the enzyme is involved in biphenyl phytoalexin biosynthesis. Pyrus pyrifolia cell cultures respond to yeast extract treatment by accumulating benzoate-derived biphenyl phytoalexins, namely, noraucuparin and aucuparin benzoate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrus pyrifolia
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 24 h, significant loss in enzyme activity (70 %) upon storage of cell-free extract Pyrus pyrifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybenzaldehyde + NAD+ + H2O 2% realtive activity as compared to benzaldehyde Pyrus pyrifolia 2-hydroxybenzoate + NADH + 2 H+
-
 ?
3-hydroxybenzaldehyde + NAD+ + H2O 1.5% realtive activity as compared to benzaldehyde Pyrus pyrifolia 3-hydroxybenzoate + NADH + 2 H+
-
 ?
4-hydroxybenzaldehyde + NAD+ + H2O 14.7% realtive activity as compared to benzaldehyde Pyrus pyrifolia 4-hydroxybenzoate + NADH + 2 H+
-
 ?
acetaldehyde + NAD+ + H2O 25.8% realtive activity as compared to benzaldehyde Pyrus pyrifolia acetate + NADH + 2 H+
-
 ?
benzaldehyde + FAD + H2O the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia benzoate + FADH2
-
 ?
benzaldehyde + FMN + H2O the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia benzoate + FMNH2
-
 ?
benzaldehyde + NAD+ + H2O the enzyme is involved in biphenyl phytoalexin biosynthesis. Pyrus pyrifolia cell cultures respond to yeast extract treatment by accumulating benzoate-derived biphenyl phytoalexins, namely, noraucuparin and aucuparin Pyrus pyrifolia benzoate + NADH + 2 H+
-
 ?
benzaldehyde + NAD+ + H2O the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia benzoate + NADH + 2 H+
-
 ?
benzaldehyde + NADP+ + H2O the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia benzoate + NADPH + 2 H+
-
 ?
propionaldehyde + NAD+ + H2O 10% realtive activity as compared to benzaldehyde Pyrus pyrifolia propionate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
benzaldehyde dehydrogenase
-
 Pyrus pyrifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pyrus pyrifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Pyrus pyrifolia

Cofactor

Cofactor Comment Organism Structure
FAD the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia
FMN the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia
NAD+ the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia
NADP+ the preferred cofactor is NAD+ followed by NADP+ (82%), FAD (40%) and FMN (34%) Pyrus pyrifolia

General Information

General Information Comment Organism
metabolism the enzyme is involved in biphenyl phytoalexin biosynthesis. Pyrus pyrifolia cell cultures respond to yeast extract treatment by accumulating benzoate-derived biphenyl phytoalexins, namely, noraucuparin and aucuparin Pyrus pyrifolia