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Purification and characterization of benzyl alcohol- and benzaldehyde-dehydrogenase from Pseudomonas putida CSV86

Shrivastava, R.; Basu, A.; Phale, P.S.; Arch. Microbiol. 193, 553-563 (2011)

Data extracted from this reference:

General Stability
General Stability
Organism
the enzyme loses its activity irreversibly when dialyzed against 50 mM potassium phosphate, pH 6.5, 5% (v/v) glycerol, 2 mM dithiothreitol without benzyl alcohol for 5 h. Enzyme activity is unaffected by varying the concentration of benzyl alcohol
Pseudomonas putida
the presence of magnesium may be essential for the stability of the enzyme
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
Pseudomonas putida
2,2'-dipyridyl
40% residual activity at 1 mM; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
Pseudomonas putida
2-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas putida
2-Methylbenzaldehyde
substrate inhibition
Pseudomonas putida
2-naphthaldehyde
substrate inhibition
Pseudomonas putida
3-Chlorobenzaldehyde
substrate inhibition
Pseudomonas putida
3-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas putida
3-Methoxybenzaldehyde
substrate inhibition
Pseudomonas putida
3-methylbenzaldehyde
substrate inhibition
Pseudomonas putida
4-Chlorobenzaldehyde
substrate inhibition
Pseudomonas putida
4-methoxybenzaldehyde
substrate inhibition
Pseudomonas putida
4-methylbenzaldehyde
substrate inhibition
Pseudomonas putida
benzaldehyde
substrate inhibition; substrate inhibition at higher concentration of benzaldehyde
Pseudomonas putida
additional information
metal chelators (10 mM) like EDTA and EGTA do not affect the activity
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
benzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0018
-
3-methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0028
-
3-Chlorobenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0028
-
3-Hydroxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.003
-
4-Chlorobenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0035
-
4-methoxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0038
-
2-naphthaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.02
-
4-methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.115
-
NAD+
apparent Km-value at substrate saturation, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.129
-
3-Methoxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.377
-
2-Methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.497
-
2-Hydroxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
5.1 mol Mg2+ per mol enzyme; tightly bound
Pseudomonas putida
additional information
BZDH shows the presence of 5.1 mol of magnesium per mole of the enzyme; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, and Zn2+ up to 2.5 mM in the assay mixture do not affect activity; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, or Zn2+ (up to 2.5 mM) does not affect the enzymatic activity; metal chelators at 10 mM like EDTA and EGTA do not affect activity
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
subunit molecular mass, determined by SDS–PAGE
Pseudomonas putida
117000
-
gel filtration; native molecular mass of BZDH, determined by Sephacryl S-300-HR gel filtration chromatography
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD+ + H2O
Pseudomonas putida
-
benzoate + NADH + H+
-
-
?
benzaldehyde + NAD+ + H2O
Pseudomonas putida CSV86
-
benzoate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
; MTCC2445
-
Pseudomonas putida CSV86
-
; MTCC2445
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration; to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.32
-
cell free extract, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
64
-
after 200fold purification, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
Storage Stability
Storage Stability
Organism
purified enzyme preparations are found to be stable and stored either at 4 or -20°C, stable up to 6 months at -20°C
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-hydroxybenzoate + NADH + H+
-
-
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
2-hydroxybenzoate + NADH + H+
-
-
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-hydroxybenzoate + NADH + 2 H+
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-methylbenzoate + NADH + 2 H+
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-methylbenzoate + NADH + H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-naphthoate + NADH + H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
naphthalene-2-carboxylic acid + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-chlorobenzoate + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-chlorobenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
3-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-hydroxybenzoate + NADH + 2 H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methoxybenzoate + NADH + H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methoxybenzoate + NADH + 2 H+
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methylbenzoate + NADH + H+
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methylbenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-chlorobenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-chlorobenzoate + NADH + H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methoxybenzoate + NADH + H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methoxybenzoate + NADH + 2 H+
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methylbenzoate + NADH + H+
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methylbenzoate + NADH + 2 H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
benzoate + NADH + 2 H+
-
-
-
ir
additional information
enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions
724140
Pseudomonas putida
?
-
-
-
?
additional information
the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde
724140
Pseudomonas putida
?
-
-
-
-
additional information
enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions
724140
Pseudomonas putida CSV86
?
-
-
-
?
additional information
the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde
724140
Pseudomonas putida CSV86
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 57000; 2 * 57000, SDS-PAGE
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14
-
benzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
16
-
3-Chlorobenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
23
-
3-methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
24
-
3-Hydroxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
33
-
2-Hydroxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
39
-
2-Methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
56
-
2-naphthaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
67
-
4-Chlorobenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
101
-
4-methoxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
120
-
4-methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
885
-
3-Methoxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.6
-
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
not active with NADP+
Pseudomonas putida
NAD+
; dependent on
Pseudomonas putida
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0013
-
3-Methoxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.003
-
4-methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.006
-
benzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.008
-
2-naphthaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.013
-
4-Chlorobenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.035
-
4-methoxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.051
-
3-methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.068
-
3-Chlorobenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.131
-
3-Hydroxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.521
-
2-Methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
1.808
-
2-Hydroxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
not active with NADP+
Pseudomonas putida
NAD+
; dependent on
Pseudomonas putida
General Stability (protein specific)
General Stability
Organism
the enzyme loses its activity irreversibly when dialyzed against 50 mM potassium phosphate, pH 6.5, 5% (v/v) glycerol, 2 mM dithiothreitol without benzyl alcohol for 5 h. Enzyme activity is unaffected by varying the concentration of benzyl alcohol
Pseudomonas putida
the presence of magnesium may be essential for the stability of the enzyme
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
Pseudomonas putida
2,2'-dipyridyl
40% residual activity at 1 mM; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions
Pseudomonas putida
2-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas putida
2-Methylbenzaldehyde
substrate inhibition
Pseudomonas putida
2-naphthaldehyde
substrate inhibition
Pseudomonas putida
3-Chlorobenzaldehyde
substrate inhibition
Pseudomonas putida
3-Hydroxybenzaldehyde
substrate inhibition
Pseudomonas putida
3-Methoxybenzaldehyde
substrate inhibition
Pseudomonas putida
3-methylbenzaldehyde
substrate inhibition
Pseudomonas putida
4-Chlorobenzaldehyde
substrate inhibition
Pseudomonas putida
4-methoxybenzaldehyde
substrate inhibition
Pseudomonas putida
4-methylbenzaldehyde
substrate inhibition
Pseudomonas putida
benzaldehyde
substrate inhibition; substrate inhibition at higher concentration of benzaldehyde
Pseudomonas putida
additional information
metal chelators (10 mM) like EDTA and EGTA do not affect the activity
Pseudomonas putida
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0013
-
3-Methoxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.003
-
4-methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.006
-
benzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.008
-
2-naphthaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.013
-
4-Chlorobenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.035
-
4-methoxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.051
-
3-methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.068
-
3-Chlorobenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.131
-
3-Hydroxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.521
-
2-Methylbenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
1.808
-
2-Hydroxybenzaldehyde
apparent Ki-value, pH 9.6, temperature not specified in the publication
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
benzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0018
-
3-methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0028
-
3-Chlorobenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0028
-
3-Hydroxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.003
-
4-Chlorobenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0035
-
4-methoxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.0038
-
2-naphthaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.02
-
4-methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.115
-
NAD+
apparent Km-value at substrate saturation, pH 9.6, temperature not specified in the publication
Pseudomonas putida
0.129
-
3-Methoxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.377
-
2-Methylbenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
0.497
-
2-Hydroxybenzaldehyde
apparent Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
5.1 mol Mg2+ per mol enzyme; tightly bound
Pseudomonas putida
additional information
BZDH shows the presence of 5.1 mol of magnesium per mole of the enzyme; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, and Zn2+ up to 2.5 mM in the assay mixture do not affect activity; external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, or Zn2+ (up to 2.5 mM) does not affect the enzymatic activity; metal chelators at 10 mM like EDTA and EGTA do not affect activity
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
subunit molecular mass, determined by SDS–PAGE
Pseudomonas putida
117000
-
gel filtration; native molecular mass of BZDH, determined by Sephacryl S-300-HR gel filtration chromatography
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD+ + H2O
Pseudomonas putida
-
benzoate + NADH + H+
-
-
?
benzaldehyde + NAD+ + H2O
Pseudomonas putida CSV86
-
benzoate + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration; to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.32
-
cell free extract, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
64
-
after 200fold purification, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
purified enzyme preparations are found to be stable and stored either at 4 or -20°C, stable up to 6 months at -20°C
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-hydroxybenzoate + NADH + H+
-
-
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
2-hydroxybenzoate + NADH + H+
-
-
-
?
2-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-hydroxybenzoate + NADH + 2 H+
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-methylbenzoate + NADH + 2 H+
-
-
-
?
2-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-methylbenzoate + NADH + H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
2-naphthoate + NADH + H+
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
naphthalene-2-carboxylic acid + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-chlorobenzoate + NADH + 2 H+
-
-
-
?
3-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-chlorobenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
3-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-hydroxybenzoate + NADH + 2 H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methoxybenzoate + NADH + H+
-
-
-
?
3-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methoxybenzoate + NADH + 2 H+
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methylbenzoate + NADH + H+
-
-
-
?
3-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
3-methylbenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-chlorobenzoate + NADH + 2 H+
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-chlorobenzoate + NADH + H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methoxybenzoate + NADH + H+
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methoxybenzoate + NADH + 2 H+
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methylbenzoate + NADH + H+
-
-
-
?
4-methylbenzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
4-methylbenzoate + NADH + 2 H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida CSV86
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
724140
Pseudomonas putida
benzoate + NADH + 2 H+
-
-
-
ir
additional information
enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions
724140
Pseudomonas putida
?
-
-
-
?
additional information
the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde
724140
Pseudomonas putida
?
-
-
-
-
additional information
enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions
724140
Pseudomonas putida CSV86
?
-
-
-
?
additional information
the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde
724140
Pseudomonas putida CSV86
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 57000; 2 * 57000, SDS-PAGE
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
14
-
benzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
16
-
3-Chlorobenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
23
-
3-methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
24
-
3-Hydroxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
33
-
2-Hydroxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
39
-
2-Methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
56
-
2-naphthaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
67
-
4-Chlorobenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
101
-
4-methoxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
120
-
4-methylbenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
885
-
3-Methoxybenzaldehyde
apparent Kcat-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.6
-
-
Pseudomonas putida
General Information
General Information
Commentary
Organism
metabolism
the wide substrate specificity of the enzyme enables the strain to detoxify methylnaphthalenes to naphthoic acids efficiently
Pseudomonas putida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the wide substrate specificity of the enzyme enables the strain to detoxify methylnaphthalenes to naphthoic acids efficiently
Pseudomonas putida
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
60
-
2-Hydroxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
110
-
2-Methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
5700
-
3-Chlorobenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
6000
-
4-methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
6800
-
3-Methoxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
9000
-
3-Hydroxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
10000
-
benzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
13000
-
3-methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
14700
-
2-naphthaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
22000
-
4-Chlorobenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
29000
-
4-methoxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
60
-
2-Hydroxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
110
-
2-Methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
5700
-
3-Chlorobenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
6000
-
4-methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
6800
-
3-Methoxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
9000
-
3-Hydroxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
10000
-
benzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
13000
-
3-methylbenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
14700
-
2-naphthaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
22000
-
4-Chlorobenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
29000
-
4-methoxybenzaldehyde
apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication; in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication
Pseudomonas putida
Other publictions for EC 1.2.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724140
Shrivastava
Purification and characterizat ...
Pseudomonas putida, Pseudomonas putida CSV86
Arch. Microbiol.
193
553-563
2011
-
-
-
-
-
2
14
12
-
2
2
2
-
2
-
-
1
-
-
-
2
1
29
1
-
-
-
11
1
-
-
2
11
-
-
-
-
-
2
-
-
2
-
14
11
12
-
2
2
2
-
-
-
1
-
-
2
1
29
1
-
-
-
11
1
-
-
-
-
1
1
-
11
11
699798
Gaid
Benzaldehyde dehydrogenase fro ...
Sorbus aucuparia
J. Plant Physiol.
166
1343-1349
2009
1
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
-
-
-
2
-
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
-
2
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
700759
Long
Involvement of snapdragon benz ...
Antirrhinum majus
Plant J.
59
256-265
2009
-
-
1
-
-
-
1
3
1
-
2
1
-
3
-
-
1
-
-
1
1
-
11
1
1
-
-
3
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
3
1
-
2
1
-
-
-
1
-
1
1
-
11
1
1
-
-
3
1
1
-
-
-
-
-
-
-
-
695093
Goettsching
Productive degradation of the ...
Acinetobacter sp. AG1
Res. Microbiol.
158
251-257
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676831
van Sint Fiet
Selection of biocatalysts for ...
Pseudomonas putida
Proc. Natl. Acad. Sci. USA
103
1693-1698
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390243
Abd El-Mawla
Benzoic acid biosynthesis in c ...
Hypericum androsaemum
Planta
214
727-733
2002
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
10
-
2
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
10
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
390242
Kim
-
DNA sequence of the phnN gene ...
Pseudomonas sp., Pseudomonas sp. DJ77
J. Microbiol.
37
224-228
1999
-
-
1
-
-
-
-
-
-
-
1
2
-
4
-
-
-
-
-
-
-
-
11
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
11
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390241
Gillooly
Molecular characterization of ...
Acinetobacter calcoaceticus
Biochem. J.
330
1375-1381
1998
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390225
Inoue
Overlapping substrate specific ...
Pseudomonas putida
J. Bacteriol.
177
1196-1201
1995
-
-
-
-
-
1
-
8
-
-
4
2
-
1
-
-
1
-
-
-
1
-
16
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
8
-
-
4
2
-
-
-
1
-
-
1
-
16
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
4814
Tsou
Mandelate pathway of Pseudomon ...
Pseudomonas putida
Biochemistry
29
9856-9862
1990
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
9
-
2
2
2
-
2
-
-
1
-
-
-
1
-
10
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
9
-
2
2
2
-
-
-
1
-
-
1
-
10
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390228
Chalmers
Purification and characterizat ...
Acinetobacter calcoaceticus
Biochem. J.
263
913-919
1989
-
-
-
-
-
-
5
3
-
1
2
1
-
1
-
-
1
-
-
-
1
-
21
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
3
-
1
2
1
-
-
-
1
-
-
1
-
21
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288330
Shaw
Physical and functional mappin ...
Pseudomonas putida
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2463-2474
1988
-
-
1
-
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1
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1
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1
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1
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1
1
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1
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-
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-
1
-
-
-
-
-
-
-
-
-
-
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-
389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
-
2
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
255
653-661
1988
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-
-
-
-
-
10
16
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1
-
1
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-
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17
-
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16
-
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1
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1
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-
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-
10
-
16
-
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1
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-
-
-
-
-
17
-
-
-
-
16
-
-
-
-
-
-
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-
390233
Chalmers
-
Quantitative immunoblotting in ...
Acinetobacter calcoaceticus
Biochem. Soc. Trans.
16
153-154
1988
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-
-
-
-
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-
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2
1
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1
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-
1
1
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-
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1
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1
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-
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-
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-
2
1
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-
-
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-
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-
1
1
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-
288331
Keil
Gene organization of the first ...
Pseudomonas putida
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169
764-770
1987
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-
1
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-
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1
-
1
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1
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-
1
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1
1
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1
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-
1
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-
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-
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-
390234
Van den Tweel
-
Physical and functional mappin ...
Flavobacterium sp.
J. Gen. Microbiol.
133
745-754
1987
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-
-
-
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1
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1
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1
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1
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1
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1
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1
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1
-
1
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390237
MacKintosh
-
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. Soc. Trans.
15
1048-1049
1987
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-
-
-
-
2
5
1
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1
1
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1
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1
-
-
-
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4
1
-
-
-
-
-
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1
-
-
-
-
-
-
1
-
-
2
-
5
-
1
-
-
1
1
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
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-
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-
-
4801
Van den Tweel
-
Microbial metabolism of D- and ...
Pseudomonas putida
Arch. Microbiol.
144
169-174
1986
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2
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1
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2
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1
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1
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-
2
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-
-
-
-
2
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-
288333
Bhat
Involvement of 4-hydroxymandel ...
Pseudomonas putida
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127
1108-1118
1976
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
1
-
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1
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-
390239
Livingstone
Two benzaldehyde dehydrogenase ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus N.C.I.B. 8250
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130
927-935
1972
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-
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2
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6
1
2
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3
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1
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6
-
2
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1
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1
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1
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2
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6
1
2
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1
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6
-
2
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1
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390240
Fewson
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Regulation of enzymes of the m ...
Acinetobacter calcoaceticus
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1968
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1
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1
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1
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1
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1
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1
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1
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1
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1
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