Literature summary for 1.2.1.28 extracted from

MacKintosh, R.W.; Fewson, C.A.
Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Substrate specificities and inhibition studies (1988), Biochem. J., 255, 653-661.

Search for more literature for 1.2.1.28

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Bromobenzaldehyde	0.1 mM, 90% inhibition, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
2-Chlorobenzaldehyde	0.1 mM, 100% inhibition, benzaldehydedehydrogenase II	Acinetobacter calcoaceticus
2-Fluorobenzaldehyde	0.1 mM, 100% inhibition, benzaldehydedehydrogenase II	Acinetobacter calcoaceticus
3-methylbenzaldehyde	0.1 mM, 75% inhibition, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
4-chloromercuribenzoate	0.005 mM, 50% inhibition after 270 min, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
4-Isopropylbenzaldehyde	0.01 mM, 87% inhibition, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
iodoacetamide	1 mM, 50% inhibition after 210 min, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
iodoacetate	10 mM, 50% inhibition after 30 min, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
N-ethylmaleimide	1 mM, 50% inhibition after 160 min, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus
pentafluorobenzaldehyde	0.1 mM, 44% inhibition, benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00057	-	benzaldehyde	-	Acinetobacter calcoaceticus
0.0015	-	cinnamaldehyde	-	Acinetobacter calcoaceticus
0.0028	-	4-Fluorobenzaldehyde	-	Acinetobacter calcoaceticus
0.0039	-	3-Fluorobenzaldehyde	-	Acinetobacter calcoaceticus
0.0051	-	thiophen-2-carboxaldehyde	-	Acinetobacter calcoaceticus
0.0065	-	3-Methoxybenzaldehyde	-	Acinetobacter calcoaceticus
0.016	-	4-methoxybenzaldehyde	-	Acinetobacter calcoaceticus
0.018	-	4-hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
0.02	-	pyridine-4-carboxaldehyde	-	Acinetobacter calcoaceticus
0.026	-	Pyridine-3-carboxaldehyde	-	Acinetobacter calcoaceticus
0.059	-	furan-2-carboxyaldehyde	-	Acinetobacter calcoaceticus
0.063	-	3-Hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
0.072	-	hexan-1-al	-	Acinetobacter calcoaceticus
0.152	-	perillaldehyde	-	Acinetobacter calcoaceticus
0.187	-	2-Hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
0.46	-	octan-1-al	-	Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
benzaldehyde + NAD+ + H2O	Acinetobacter calcoaceticus	-	benzoate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-hydroxybenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	2-hydroxybenzoate + NADH + H+	-	?
3-fluorobenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	3-fluorobenzoate + NADH + H+	-	?
3-hydroxybenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	3-hydroxybenzoate + NADH + H+	-	?
3-methoxybenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	3-methoxybenzoate + NADH	-	?
4-fluorobenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	4-fluorobenzoate + NADH + H+	-	?
4-hydroxybenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	4-hydroxybenzoate + NADH + H+	-	?
4-methoxybenzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	4-methoxybenzoate + NADH + H+	-	?
benzaldehyde + NAD+ + H2O	-	Acinetobacter calcoaceticus	benzoate + NADH + H+	-	?
benzaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II exhibits esterase activity with 4-nitrophenyl acetate	Acinetobacter calcoaceticus	benzoate + NADH + H+	-	?
cinnamaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	cinnamate + NADH + H+	-	?
furan-2-carboxyaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	furan-2-formiate + NADH	-	?
hexan-1-al + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	hexanoate + NADH	-	?
octan-1-al + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	octanoate + NADH	-	?
perillaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	perillic acid + NADH	-	?
pyridin-3-carboxyaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	pyridin-3-formiate + NADH	-	?
pyridine 4-carboxyaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	pyridine 4-formiate + NADH	-	?
thiophen-2-carboxaldehyde + NAD+ + H2O	-	Acinetobacter calcoaceticus	thiophen-2-formiate + NADH	-	r
thiophen-2-carboxyaldehyde + NAD+ + H2O	benzaldehyde dehydrogenase II	Acinetobacter calcoaceticus	thiophen-2-formiate + NADH	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17	-	perillaldehyde	-	Acinetobacter calcoaceticus
20	-	4-hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
26	-	cinnamaldehyde	-	Acinetobacter calcoaceticus
26	-	octan-1-al	-	Acinetobacter calcoaceticus
29	-	3-Hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
29	-	hexan-1-al	-	Acinetobacter calcoaceticus
33	-	3-Methoxybenzaldehyde	-	Acinetobacter calcoaceticus
41	-	2-Hydroxybenzaldehyde	-	Acinetobacter calcoaceticus
41	-	3-Fluorobenzaldehyde	-	Acinetobacter calcoaceticus
46	-	furan-2-carboxaldehyde	-	Acinetobacter calcoaceticus
49	-	4-methoxybenzaldehyde	-	Acinetobacter calcoaceticus
52	-	thiophen-2-carboxyaldehyde	-	Acinetobacter calcoaceticus
64	-	4-Fluorobenzaldehyde	-	Acinetobacter calcoaceticus
66	-	pyridine-4-carboxaldehyde	-	Acinetobacter calcoaceticus
68	-	Pyridine-3-carboxaldehyde	-	Acinetobacter calcoaceticus
72	-	benzaldehyde	-	Acinetobacter calcoaceticus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Acinetobacter calcoaceticus

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Release 2023.1 (January 2023)