Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-galactarate | induces KGSADH-II | Azospirillum brasilense | |
D-glucarate | induces KGSADH-II | Azospirillum brasilense | |
hydroxy-L-proline | induces KGSADH-III | Azospirillum brasilense | |
L-arabinose | induces KGSADH-I | Azospirillum brasilense |
Application | Comment | Organism |
---|---|---|
additional information | the ALDH branch including ycbD protein is designated the KGSADH subclass (type III) | Bacillus subtilis |
additional information | three different types of KGSADH appear in the bacterial evolutional stage convergently | Azospirillum brasilense |
Cloned (Comment) | Organism |
---|---|
expressed as His6-tagged ycbD protein | Bacillus subtilis |
KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha | Azospirillum brasilense |
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha | Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Azospirillum brasilense | |
0.0075 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Azospirillum brasilense | |
0.011 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Azospirillum brasilense | |
0.0149 | - |
NADP+ | - |
Azospirillum brasilense | |
0.0246 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Azospirillum brasilense | |
0.0352 | - |
Octylaldehyde | - |
Bacillus subtilis | |
0.0393 | - |
heptaldehyde | - |
Bacillus subtilis | |
0.0622 | - |
NADP+ | - |
Azospirillum brasilense | |
0.0814 | - |
hexylaldehyde | - |
Bacillus subtilis | |
0.094 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Bacillus subtilis | |
0.1169 | - |
NAD+ | - |
Azospirillum brasilense | |
0.145 | - |
Valeraldehyde | - |
Bacillus subtilis | |
0.17 | - |
butylaldehyde | - |
Bacillus subtilis | |
0.2599 | - |
NAD+ | KGSADH-I | Azospirillum brasilense | |
0.5569 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Bacillus subtilis | |
0.74 | - |
NAD+ | - |
Bacillus subtilis | |
1.08 | - |
NADP+ | - |
Bacillus subtilis | |
1.136 | - |
NAD+ | - |
Azospirillum brasilense | |
1.46 | - |
propionaldehyde | - |
Bacillus subtilis | |
2.427 | - |
NADP+ | KGSADH-I | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
gel filtration | Azospirillum brasilense |
54351 | - |
2 * 57000 or 2 * 54351, gel filtration | Azospirillum brasilense |
55185 | - |
2 * 57000 or 2 * 55185, gel filtration | Azospirillum brasilense |
57000 | - |
2 * 57000 or 2 * 54351, gel filtration | Azospirillum brasilense |
57000 | - |
2 * 57000 or 2 * 55185, gel filtration | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | Q08IB7 | strain ATCC29145 | - |
Azospirillum brasilense | Q08IC0 | strain ATCC29145 | - |
Azospirillum brasilense | Q1JUP4 | strain ATCC29145 | - |
Azospirillum brasilense ATCC 29145 | Q08IB7 | strain ATCC29145 | - |
Azospirillum brasilense ATCC 29145 | Q08IC0 | strain ATCC29145 | - |
Azospirillum brasilense ATCC 29145 | Q1JUP4 | strain ATCC29145 | - |
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration | Azospirillum brasilense |
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration | Azospirillum brasilense |
Storage Stability | Organism |
---|---|
-35°C, buffer E, pH 8.0, 250 mM imidazole | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dioxopentanoate + NAD+ + H2O | - |
Bacillus subtilis | 2-oxoglutarate + NADH + H+ | - |
r | |
2,5-dioxopentanoate + NAD+ + H2O | - |
Azospirillum brasilense | 2-oxoglutarate + NADH + H+ | - |
r | |
2,5-dioxopentanoate + NAD+ + H2O | - |
Azospirillum brasilense ATCC 29145 | 2-oxoglutarate + NADH + H+ | - |
r | |
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | alpha-ketoglutarate + NAD(P)H + H+ | - |
? | |
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O | - |
Azospirillum brasilense | alpha-ketoglutarate + NAD(P)H + H+ | - |
? | |
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O | - |
Azospirillum brasilense ATCC 29145 | alpha-ketoglutarate + NAD(P)H + H+ | - |
? | |
butyraldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | n-butanoate + NAD(P)H + H+ | - |
? | |
heptaldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | n-heptanoate + NAD(P)H + H+ | - |
? | |
hexylaldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | n-hexanoate + NAD(P)H + H+ | - |
? | |
octylaldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | n-octanoate + NAD(P)H + H+ | - |
? | |
propionaldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | propionate + NAD(P)H + H+ | - |
? | |
valeraldehyde + NAD(P)+ + H2O | - |
Bacillus subtilis | n-pentanoate + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000 or 2 * 54351, gel filtration | Azospirillum brasilense |
dimer | 2 * 57000 or 2 * 55185, gel filtration | Azospirillum brasilense |
tetramer | gel filtration | Bacillus subtilis |
tetramer | gel filtration | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
alpha-ketoglutaric semialdehyde dehydrogenase | - |
Bacillus subtilis |
alpha-ketoglutaric semialdehyde dehydrogenase | - |
Azospirillum brasilense |
alphaKGSA dehydrogenase | - |
Bacillus subtilis |
alphaKGSA dehydrogenase | - |
Azospirillum brasilense |
KGSADH | - |
Bacillus subtilis |
KGSADH-I | - |
Azospirillum brasilense |
KGSADH-II | - |
Azospirillum brasilense |
KGSADH-III | - |
Azospirillum brasilense |
ycbD protein | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
NAD+ | - |
Bacillus subtilis | |
0.87 | - |
heptaldehyde | - |
Bacillus subtilis | |
0.97 | - |
Octylaldehyde | - |
Bacillus subtilis | |
1.08 | - |
NADP+ | - |
Bacillus subtilis | |
1.12 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Bacillus subtilis | |
1.29 | - |
propionaldehyde | - |
Bacillus subtilis | |
1.35 | - |
butylaldehyde | - |
Bacillus subtilis | |
1.47 | - |
hexylaldehyde | - |
Bacillus subtilis | |
2.1 | - |
Valeraldehyde | - |
Bacillus subtilis | |
2.75 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Bacillus subtilis | |
9.86 | - |
NADP+ | KGSADH-I | Azospirillum brasilense | |
10.2 | - |
NADP+ | - |
Azospirillum brasilense | |
15.3 | - |
NADP+ | - |
Azospirillum brasilense | |
15.4 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Azospirillum brasilense | |
16.7 | - |
NAD+ | KGSADH-I | Azospirillum brasilense | |
21.3 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Azospirillum brasilense | |
23 | - |
alpha-Ketoglutaric semialdehyde | with NADP+ as cofactor | Azospirillum brasilense | |
35.5 | - |
NAD+ | - |
Azospirillum brasilense | |
52.3 | - |
NAD+ | - |
Azospirillum brasilense | |
53.9 | - |
alpha-Ketoglutaric semialdehyde | with NAD+ as cofactor | Azospirillum brasilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Azospirillum brasilense | |
NAD+ | no significant preference between NAD+ and NADP+ in activity | Azospirillum brasilense | |
NAD+ | prefered cofactor of KGSADH-II | Azospirillum brasilense | |
NADP+ | - |
Bacillus subtilis | |
NADP+ | - |
Azospirillum brasilense | |
NADP+ | prefered cofactor of KGSADH-III | Azospirillum brasilense |