Cloned (Comment) | Organism |
---|---|
vectors containing His-Tag constructs overexpressed in Escherichia coli BL21 (DE3)pLysS strain | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F180T | renders an enzyme with the ability to use NADP+. NADP+ activity is higher than that attained with NAD+. Exhibits a 16fold increase in the Vm/Km ratio with NAD+ as the coenzyme. Absence of Mg2+ inhibitory effect on F180T activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NAD+ | mutant F180T | Escherichia coli | |
0.0078 | - |
NADP+ | mutant F180T | Escherichia coli | |
0.047 | - |
NAD+ | wild-type | Escherichia coli | |
0.25 | - |
propionaldehyde | wild-type | Escherichia coli | |
0.26 | - |
propionaldehyde | mutant F180T | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant F180T purified to homogeneity (more than 95% pure), on Ni2+ resin | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 100 mM H2NaPO4, pH 7.5, 100 mM NaCl,0.025% 2-mercaptoethanol, 50% glycerol, stable for 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Escherichia coli | benzoate + NADH + H+ | - |
? | |
p-methoxybenzaldehyde + NAD+ + H2O | - |
Escherichia coli | p-methoxybenzoate + NADH + H+ | - |
? | |
p-nitrobenzaldehyde + NAD+ + H2O | - |
Escherichia coli | p-nitrobenzoate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Escherichia coli | propionate + NADH + H+ | - |
? | |
propionaldehyde + NADP+ + H2O | ALD wild-type only uses NAD+. F180T mutation renders an enzyme with the ability to use NADP+ | Escherichia coli | propionate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | ALD only uses NAD+. Residue F180 of ALD may participate in coenzyme specificity | Escherichia coli |