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Literature summary for 1.2.1.22 extracted from

  • Grochowski, L.L.; Xu, H.; White, R.H.
    Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis (2006), J. Bacteriol., 188, 2836-2844.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
iodacetamide
-
 Methanocaldococcus jannaschii
N-ethylmaleimide
-
 Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.284
-
 propionaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii
0.302
-
 D-Lactaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 SDS-PAGE Methanocaldococcus jannaschii
180000
-
 gel-filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58806
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography Methanocaldococcus jannaschii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Methanocaldococcus jannaschii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O 25% activity compared to D-lactaldehyde Methanocaldococcus jannaschii acetate + NADH + H+
-
 ?
acrylaldehyde + NAD+ + H2O 19% activity compared to D-lactaldehyde Methanocaldococcus jannaschii acrylate + NADH
-
 ?
crotonaldehyde + NAD+ + H2O 84% activity compared to D-lactaldehyde Methanocaldococcus jannaschii crotonate + NADH
-
 ?
D-lactaldehyde + NAD+ + H2O
-
 Methanocaldococcus jannaschii D-lactate + NADH + H+
-
 ?
DL-glyceraldehyde + NAD+ + H2O 97% activity compared to D-lactaldehyde Methanocaldococcus jannaschii glycerate + NADH
-
 ?
formaldehyde + NAD+ + H2O 4% activity compared to D-lactaldehyde Methanocaldococcus jannaschii formate + NADH
-
 ?
glycolaldehyde + NAD+ + H2O 35% activity compared to D-lactaldehyde Methanocaldococcus jannaschii glycolate + NADH
-
 ?
propionaldehyde + NAD+ + H2O 187% activity compared to D-lactaldehyde Methanocaldococcus jannaschii propionate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 51000, gel-filtration Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
Lactaldehyde dehydrogenase
-
 Methanocaldococcus jannaschii
MJ1411
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 86% activity after 80°C for 10 min Methanocaldococcus jannaschii
90
-
 45% activity after 90°C for 10 min Methanocaldococcus jannaschii
100
-
 25% activity after 100°C for 10 min Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 D-Lactaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii
3
-
 propionaldehyde 100 mM TES pH 7.5, 2.5 mM NAD+ Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
7 10 17% activity at pH 7.0, almost 100% activity at pH 10.0 Methanocaldococcus jannaschii