BRENDA - Enzyme Database
show all sequences of 1.2.1.21

Separation and characterization of glycolaldehyde dehydrogenase isozymes in Escherichia coli B

Tani, Y.; Morita, H.; Nishise, H.; Ogata, K.; Agric. Biol. Chem. 42, 63-68 (1978)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
1 mM, isozyme A, 26% inhibition
Escherichia coli
As3+
1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Ca2+
1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Co2+
1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Fe2+
; 1 mM, isozyme A, 18% inhibition
Escherichia coli
HgCl2
1 mM, complete inhibition of isozymes A, B, and C
Escherichia coli
KCN
isozymes A and C
Escherichia coli
Mg2+
1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Mn2+
1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition
Escherichia coli
monoiodoacetate
1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition
Escherichia coli
MoO22-
1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Zn2+
1 mM, isozyme A, 30% inhibition
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
isozyme B, gel filtration
Escherichia coli
225000
-
isozyme A, gel filtration
Escherichia coli
345000
-
isozyme C, gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli B WG1
-
-
-
Purification (Commentary)
Commentary
Organism
partial: isozymes A, B and C
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7
-
isozyme B
Escherichia coli
2.75
-
isozyme C
Escherichia coli
3.66
-
isozyme A
Escherichia coli
6.38
-
isozyme A from pyridoxine auxotroph strain WG3
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli
acetate + NADH + H+
-
390215
Escherichia coli
r
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli B WG1
acetate + NADH + H+
-
390215
Escherichia coli B WG1
r
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli
benzoate + NADH + H+
-
390215
Escherichia coli
-
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli B WG1
benzoate + NADH + H+
-
390215
Escherichia coli B WG1
-
betaine + NADH
isozyme A 139% of activity with glycolate, isozyme B, 76%
390215
Escherichia coli
betaine aldehyde + NAD+ + H2O
glucono-delta-lactone is not used as substrate
390215
Escherichia coli
?
formate + NADH
isozyme A, 163% of activity with glycolate
390215
Escherichia coli
methanal + NAD+
-
390215
Escherichia coli
?
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli
glycolate + NADH
-
390215
Escherichia coli
r
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli B WG1
glycolate + NADH
-
390215
Escherichia coli B WG1
r
glyoxylate + NADH
isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150%
390215
Escherichia coli
glyoxal + NAD+
-
390215
Escherichia coli
?
malonate + NADH
isozyme A, 83% of activity with glycolate
390215
Escherichia coli
malonic semialdehyde + NAD+ + H2O
-
-
-
?
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli
glyoxylate + NAD+
-
390215
Escherichia coli
?
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli B WG1
glyoxylate + NAD+
-
390215
Escherichia coli B WG1
?
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli
succinic semialdehyde + NAD+ + H2O
-
-
-
?
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli B WG1
succinic semialdehyde + NAD+ + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
isozyme A
Escherichia coli
30
-
isozyme A from strain WG3
Escherichia coli
45
-
isozymes B and C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
glycolate reduction, isozyme A, B
Escherichia coli
5.4
-
isozyme A, glycolaldehyde oxidation
Escherichia coli
6
-
glycolate reduction, isozyme C
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
1 mM, isozyme A, 26% inhibition
Escherichia coli
As3+
1 mM, isozyme A, 42% inhibition, isozyme B, 11% inhibition, isozyme C, 55% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Ca2+
1 mM, isozyme A, 42% inhibition, isozyme B, 32% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Co2+
1 mM, isozyme A, 71% inhibition, isozyme B, 51% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Fe2+
; 1 mM, isozyme A, 18% inhibition
Escherichia coli
HgCl2
1 mM, complete inhibition of isozymes A, B, and C
Escherichia coli
KCN
isozymes A and C
Escherichia coli
Mg2+
1 mM, isozyme A, 33% inhibition, isozyme B, 54% inhibition, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Mn2+
1 mM, isozyme A, 20% inhibition, isozyme C, 44% inhibition
Escherichia coli
monoiodoacetate
1 mM, isozymes A, 83% inhibition, isozyme C, 100% inhibition
Escherichia coli
MoO22-
1 mM, complete inhibition of isozyme A from pyridoxine auxotroph mutant strain WG3
Escherichia coli
Zn2+
1 mM, isozyme A, 30% inhibition
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
isozyme B, gel filtration
Escherichia coli
225000
-
isozyme A, gel filtration
Escherichia coli
345000
-
isozyme C, gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
partial: isozymes A, B and C
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7
-
isozyme B
Escherichia coli
2.75
-
isozyme C
Escherichia coli
3.66
-
isozyme A
Escherichia coli
6.38
-
isozyme A from pyridoxine auxotroph strain WG3
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli
acetate + NADH + H+
-
390215
Escherichia coli
r
acetaldehyde + NAD+ + H2O
reverse reaction, isozyme A, 188% of activity with glycolate, isozyme B, 112%, isozyme C, 74%
390215
Escherichia coli B WG1
acetate + NADH + H+
-
390215
Escherichia coli B WG1
r
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli
benzoate + NADH + H+
-
390215
Escherichia coli
-
benzaldehyde + NAD+ + H2O
isozyme A, 195% of activity with glycolate in reverse reaction
390215
Escherichia coli B WG1
benzoate + NADH + H+
-
390215
Escherichia coli B WG1
-
betaine + NADH
isozyme A 139% of activity with glycolate, isozyme B, 76%
390215
Escherichia coli
betaine aldehyde + NAD+ + H2O
glucono-delta-lactone is not used as substrate
390215
Escherichia coli
?
formate + NADH
isozyme A, 163% of activity with glycolate
390215
Escherichia coli
methanal + NAD+
-
390215
Escherichia coli
?
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli
glycolate + NADH
-
390215
Escherichia coli
r
glycolaldehyde + NAD+ + H2O
-
390215
Escherichia coli B WG1
glycolate + NADH
-
390215
Escherichia coli B WG1
r
glyoxylate + NADH
isozyme A, 417% of activity with glycolate, isozyme B 714%, isozyme C 150%
390215
Escherichia coli
glyoxal + NAD+
-
390215
Escherichia coli
?
malonate + NADH
isozyme A, 83% of activity with glycolate
390215
Escherichia coli
malonic semialdehyde + NAD+ + H2O
-
-
-
?
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli
glyoxylate + NAD+
-
390215
Escherichia coli
?
oxalate + NADH
isozyme A, 167% of activity with glycolate, isozyme B, 71%
390215
Escherichia coli B WG1
glyoxylate + NAD+
-
390215
Escherichia coli B WG1
?
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli
succinic semialdehyde + NAD+ + H2O
-
-
-
?
succinate + NADH
isozyme A, 146% of activity with glycolate, isozyme B 6%
390215
Escherichia coli B WG1
succinic semialdehyde + NAD+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
isozyme A
Escherichia coli
30
-
isozyme A from strain WG3
Escherichia coli
45
-
isozymes B and C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
glycolate reduction, isozyme A, B
Escherichia coli
5.4
-
isozyme A, glycolaldehyde oxidation
Escherichia coli
6
-
glycolate reduction, isozyme C
Escherichia coli
Other publictions for EC 1.2.1.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288131
Caballero
Identification of lactaldehyde ...
Escherichia coli
J. Biol. Chem.
258
7788-7792
1983
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3
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1
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7
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1
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1
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7
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1
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390210
Boronat
Experimental evolution of a me ...
Escherichia coli
J. Bacteriol.
153
134-139
1983
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2
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3
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1
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2
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2
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3
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1
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390211
Morita
-
Glycolaldehyde dehydrogenase, ...
Bacillus subtilis, Bacillus subtilis IFO 3007
Agric. Biol. Chem.
43
317-323
1979
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2
3
4
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2
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1
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1
1
12
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2
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2
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2
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3
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4
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1
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1
1
12
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-
390212
Morita
-
Bacterial distribution of glyc ...
Agrobacterium tumefaciens, Alcaligenes viscolactis, Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Flavobacterium sp., Klebsiella aerogenes, Klebsiella pneumoniae, Micrococcus luteus, Pimelobacter simplex, Protaminobacter alboflavus, Proteus vulgaris, Pseudomonas fluorescens, Rhodococcus hoagii, Salmonella enterica subsp. enterica serovar Typhimurium, Staphylococcus aureus
Agric. Biol. Chem.
43
185-186
1979
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17
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19
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18
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19
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18
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-
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-
390213
Morita
-
Further characterization of gl ...
Escherichia coli, Escherichia coli B WG1
Agric. Biol. Chem.
42
2077-2081
1978
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-
-
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2
16
2
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4
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4
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6
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2
6
16
2
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4
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-
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-
-
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-
390214
Morita
-
Control of glycolaldehyde dehy ...
Escherichia coli, Escherichia coli B WG1
Agric. Biol. Chem.
42
69-73
1978
3
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5
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4
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2
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1
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3
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5
1
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2
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-
-
-
-
390215
Tani
-
Separation and characterizatio ...
Escherichia coli, Escherichia coli B WG1
Agric. Biol. Chem.
42
63-68
1978
-
-
-
-
-
-
12
-
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-
3
-
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4
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-
1
-
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4
1
14
-
3
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3
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1
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1
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12
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3
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1
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4
1
14
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3
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3
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