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Literature summary for 1.2.1.19 extracted from

  • Jorge, J.M.; Leggewie, C.; Wendisch, V.F.
    A new metabolic route for the production of gamma-aminobutyric acid by Corynebacterium glutamicum from glucose (2016), Amino Acids, 48, 2519-2531 .
    View publication on PubMed
Search for more literature for 1.2.1.19

Application

Application Comment Organism
synthesis the enzyme is useful for GABA production in an engineered strain of Corynebacterium glutamicum produces 5.3 g/l of GABA Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene patD, recombinant expression in Corynebacterium glutamicum strain ATCC 13032 with deleted gabTDP operon and cgmA gene Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information a putrescine-producing recombinant Corynebacterium glutamicum strain is converted into a 4-aminobutyric acid, GABA, producing strain by heterologous expression of putrescine transaminase (PatA) and gamma-aminobutyraldehyde dehydrogenase (PatD) genes from Escherichia coli. The resultant strain produces 5.3 g/l of GABA. GABA production is improved further by adjusting the concentration of nitrogen in the culture medium, by avoiding the formation of the by-product N-acetylputrescine and by deletion of the genes for GABA catabolism and GABA re-uptake Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information addition of 4-aminobutyric acid, GABA, to the growth medium hardly affects growth of Corynebacterium glutamicum, since a half-inhibitory concentration of 1.1 M GABA is determined Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanal + NAD+ + H2O Escherichia coli
-
 4-aminobutanoate + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 MG1655
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanal + NAD+ + H2O
-
 Escherichia coli 4-aminobutanoate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
gamma-aminobutyraldehyde dehydrogenase
-
 Escherichia coli
PatD
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism as alternative to GABA production by glutamate decarboxylation, another route for the production of GABA via putrescine is established in Corynebacterium glutamicum Escherichia coli