BRENDA - Enzyme Database
show all sequences of 1.2.1.17

Carbon assimilation by Pseudomonas oxalaticus (OX1)

Quayle, J.R.; Taylor, G.A.; Biochem. J. 78, 611-615 (1961)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Tris buffer
-
Cupriavidus oxalaticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + CoA + NADP+
Pseudomonas sp.
-
oxalyl-CoA + NADPH
-
Pseudomonas sp.
r
glyoxylate + CoA + NADP+
Cupriavidus oxalaticus
-
oxalyl-CoA + NADPH
-
Cupriavidus oxalaticus
r
Organism
Organism
UniProt
Commentary
Textmining
Cupriavidus oxalaticus
-
-
-
Pseudomonas sp.
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose
Cupriavidus oxalaticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
-
Cupriavidus oxalaticus
7.2
-
-
Cupriavidus oxalaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
glyoxylate + CoA + NADP+
-
390208
Pseudomonas sp.
oxalyl-CoA + NADPH
-
390208
Pseudomonas sp.
r
glyoxylate + CoA + NADP+
-
390208
Cupriavidus oxalaticus
oxalyl-CoA + NADPH
-
390208
Cupriavidus oxalaticus
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
glyoxylate + CoA + NADP+
Cupriavidus oxalaticus
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Pseudomonas sp.
CoA
specific for
Cupriavidus oxalaticus
NADP+
-
Pseudomonas sp.
NADP+
specific for
Cupriavidus oxalaticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Pseudomonas sp.
CoA
specific for
Cupriavidus oxalaticus
NADP+
-
Pseudomonas sp.
NADP+
specific for
Cupriavidus oxalaticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Tris buffer
-
Cupriavidus oxalaticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
glyoxylate + CoA + NADP+
Pseudomonas sp.
-
oxalyl-CoA + NADPH
-
Pseudomonas sp.
r
glyoxylate + CoA + NADP+
Cupriavidus oxalaticus
-
oxalyl-CoA + NADPH
-
Cupriavidus oxalaticus
r
Purification (Commentary) (protein specific)
Commentary
Organism
using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose
Cupriavidus oxalaticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
-
Cupriavidus oxalaticus
7.2
-
-
Cupriavidus oxalaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
glyoxylate + CoA + NADP+
-
390208
Pseudomonas sp.
oxalyl-CoA + NADPH
-
390208
Pseudomonas sp.
r
glyoxylate + CoA + NADP+
-
390208
Cupriavidus oxalaticus
oxalyl-CoA + NADPH
-
390208
Cupriavidus oxalaticus
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
glyoxylate + CoA + NADP+
Cupriavidus oxalaticus
Other publictions for EC 1.2.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725272
Schneider
Oxalyl-coenzyme A reduction to ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
J. Bacteriol.
194
3144-3155
2012
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-
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-
2
-
18
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-
-
-
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2
-
2
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700908
Singh
An ATP and oxalate generating ...
Pseudomonas fluorescens
PLoS ONE
4
e7344
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390204
Paster
-
The possible role of some enzy ...
Aspergillus ochraceus
Can. J. Microbiol.
29
718-723
1983
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
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-
-
-
1
-
2
-
-
-
-
-
-
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-
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2
-
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-
2
-
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-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390205
Baqui
-
Glyoxylate metabolism and fatt ...
Mangifera indica
Phytochemistry
16
51-54
1977
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
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-
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-
2
-
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-
2
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-
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-
1
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2
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-
-
-
-
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-
-
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-
-
-
-
390206
Chandra
Oxalate and formate metabolism ...
Alcaligenes sp., Pseudomonas sp.
Antonie van Leeuwenhoek
41
465-477
1975
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
4
-
2
-
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390207
Quayle
-
Glyoxylate dehydrogenase ...
Cupriavidus oxalaticus, Pseudomonas sp.
Methods Enzymol.
9
342-346
1966
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-
-
-
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-
1
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-
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-
2
-
2
-
-
1
-
-
-
2
2
2
-
1
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-
-
-
1
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4
-
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-
-
4
-
-
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-
1
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2
-
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-
1
-
-
2
2
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390208
Quayle
Carbon assimilation by Pseudom ...
Cupriavidus oxalaticus, Pseudomonas sp.
Biochem. J.
78
611-615
1961
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-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
1
-
-
-
2
-
2
-
-
-
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-
-
1
-
-
4
-
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4
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1
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2
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1
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2
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2
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1
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