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Literature summary for 1.2.1.16 extracted from

  • Mayer, J.; Cook, A.M.
    Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway (2009), J. Bacteriol., 191, 6052-6058.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.16

Activating Compound

Activating Compound Comment Organism Structure
4-aminobutyrate induction during growth with 4-aminobutyrate (GABA) Cupriavidus necator

General Stability

General Stability Organism
enzyme is labile (50% loss of activity overnight) but can be largely stabilized (in crude extract only) by the addition of 10 mM dithiothreitol 1.2.1.16 Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 3-sulfopropanal Km apparent value Cupriavidus necator
0.23
-
 NAD+ Km apparent value Cupriavidus necator
0.35
-
 NADP+ Km apparent value Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-sulfopropanal + NAD(P)+ + H2O Cupriavidus necator
-
 3-sulfopropanoate + NAD(P)H + 2 H+
-
 ?
succinate semialdehyde + NAD(P)+ + H2O Cupriavidus necator
-
 succinate + NAD(P)H + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K2K1
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation and anion exchange chromatography and gel filtration Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of 0.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
additional information
-
 specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator
additional information
-
 specific activity of 0.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
additional information
-
 specific activity of 0.6 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with ammonium as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
additional information
-
 specific activity of 0.7 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
additional information
-
 specific activity of 10 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
additional information
-
 specific activity of 2.5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NADP+ Cupriavidus necator
additional information
-
 specific activity of 3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
additional information
-
 specific activity of 5 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is sulfopropanal and NAD+ Cupriavidus necator
additional information
-
 specific activity of 5.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with 4-aminobutyrate as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator
additional information
-
 specific activity of 7.0 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NADP+ Cupriavidus necator
additional information
-
 specific activity of 7.3 mkat/kg protein is detected in crude cell extracts of Cupriavidus necator grown with homotaurine as the sole nitrogen source, substrate is succinale semialdehyde and NAD+ Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-sulfopropanal + NAD(P)+ + H2O
-
 Cupriavidus necator 3-sulfopropanoate + NAD(P)H + 2 H+
-
 ?
succinate semialdehyde + NAD(P)+ + H2O
-
 Cupriavidus necator succinate + NAD(P)H + 2 H+
-
 ?
succinate semialdehyde + NAD+ + H2O
-
 Cupriavidus necator succinate + NADH + H+
-
 r

Subunits

Subunits Comment Organism
homodimer
-
 Cupriavidus necator

Synonyms

Synonyms Comment Organism
3-sulfopropanal dehydrogenase
-
 Cupriavidus necator
GabD1
-
 Cupriavidus necator
NAD(P)+-coupled 3-sulfopropanal dehydrogenase
-
 Cupriavidus necator
succinate-semialdehyde dehydrogenase
-
 Cupriavidus necator
sulfopropanal dehydrogenase
-
 Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 specific activity increases steadily to a broad optimum Cupriavidus necator

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 enzyme is almost inactive Cupriavidus necator

Expression

Organism Comment Expression
Cupriavidus necator transcript is detected inducibly in both homotaurine and GABA-grown cells up

General Information

General Information Comment Organism
metabolism GABA metabolism Cupriavidus necator