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show all sequences of 1.2.1.15

X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC

Wilding, M.; Scott, C.; Peat, T.S.; Newman, J.; Acta Crystallogr. Sect. F 73, 24-28 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene KES23460, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas sp.
Crystallization (Commentary)
Crystallization
Organism
purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
A0A081YAY7
-
-
Pseudomonas sp. AAC
A0A081YAY7
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity
Pseudomonas sp.
Subunits
Subunits
Commentary
Organism
hexamer
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene KES23460, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant enzyme, method screening, crystallization of 20 mg/ml protein with crystalliztaion solution consisting of 1.38 M ammonium sulfate, 3.35% 2,2,2-trifluoroethanol, 2% benzamidine, the resulting crystals are used for microseding with 5 mg/ml protein, 1 mM acetyl-CoA, and a solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, at 8C, method optimization, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement using structure with PDB ID 4zz7 as the search model
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity
Pseudomonas sp.
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
-
Pseudomonas sp.
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA
Pseudomonas sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the beta-alanine catabolism to produce acetyl-CoA
Pseudomonas sp.
Other publictions for EC 1.2.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741539
Wilding
X-ray crystal structure of a ...
Pseudomonas sp., Pseudomonas sp. AAC
Acta Crystallogr. Sect. F
73
24-28
2017
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1
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684557
Yebra
Identification of a gene clust ...
Lactobacillus casei
Appl. Environ. Microbiol.
73
3850-3858
2007
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1
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348638
Nakamura
Studies on malonic semialdehyd ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
50
147-152
1961
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2
10
2
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2
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10
3
2
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1
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3
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1
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