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show all sequences of 1.2.1.15

Studies on malonic semialdehyde dehydrogenase from Pseudomonas aeruginosa

Nakamura, K.; Bernheim, F.; Biochim. Biophys. Acta 50, 147-152 (1961)

Data extracted from this reference:

General Stability
General Stability
Organism
1 mM mercaptoethanol is essential for stability
Pseudomonas aeruginosa
unstable in dilute solution
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Pseudomonas aeruginosa
Cu2+
-
Pseudomonas aeruginosa
glyoxylate
competitive inhibitor
Pseudomonas aeruginosa
HgCl2
-
Pseudomonas aeruginosa
malonate
competitive inhibitor
Pseudomonas aeruginosa
Ni2+
-
Pseudomonas aeruginosa
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
phosphate
-
Pseudomonas aeruginosa
succinate semialdehyde
non-competitive inhibitor
Pseudomonas aeruginosa
Zn2+
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
Malonate semialdehyde
-
Pseudomonas aeruginosa
0.11
-
NAD+
-
Pseudomonas aeruginosa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
increases activity 42% at 1 mM
Pseudomonas aeruginosa
Mg2+
increases activity 64% at 1 mM
Pseudomonas aeruginosa
Mn2+
increases activity 27% at 1 mM
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-oxopropanoate + NAD+ + H2O
Pseudomonas aeruginosa
-
malonate + NADH
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.203
-
-
Pseudomonas aeruginosa
Storage Stability
Storage Stability
Organism
-20°C, purified enzyme, 1 month
Pseudomonas aeruginosa
0°C, bovine serum albumin, NAD+, 1 week
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-oxopropanoate + NAD+ + H2O
-
348638
Pseudomonas aeruginosa
malonate + NADH
-
-
-
-
additional information
no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde
348638
Pseudomonas aeruginosa
?
-
-
-
-
NAD(P)+ + 3-oxopropanoate + H2O
-
348638
Pseudomonas aeruginosa
malonate + NAD(P)H
-
348638
Pseudomonas aeruginosa
ir
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
with NAD+ and bovine serum albumin at pH 8.7
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
malonate semialdehyde + NAD+
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
NADP+
less than 10% activity compared with NAD+
Pseudomonas aeruginosa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.56
-
succinate semialdehyde
-
Pseudomonas aeruginosa
0.57
-
malonate
-
Pseudomonas aeruginosa
1.8
-
glyoxylate
-
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas aeruginosa
NADP+
less than 10% activity compared with NAD+
Pseudomonas aeruginosa
General Stability (protein specific)
General Stability
Organism
1 mM mercaptoethanol is essential for stability
Pseudomonas aeruginosa
unstable in dilute solution
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Pseudomonas aeruginosa
Cu2+
-
Pseudomonas aeruginosa
glyoxylate
competitive inhibitor
Pseudomonas aeruginosa
HgCl2
-
Pseudomonas aeruginosa
malonate
competitive inhibitor
Pseudomonas aeruginosa
Ni2+
-
Pseudomonas aeruginosa
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
phosphate
-
Pseudomonas aeruginosa
succinate semialdehyde
non-competitive inhibitor
Pseudomonas aeruginosa
Zn2+
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.56
-
succinate semialdehyde
-
Pseudomonas aeruginosa
0.57
-
malonate
-
Pseudomonas aeruginosa
1.8
-
glyoxylate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
Malonate semialdehyde
-
Pseudomonas aeruginosa
0.11
-
NAD+
-
Pseudomonas aeruginosa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
increases activity 42% at 1 mM
Pseudomonas aeruginosa
Mg2+
increases activity 64% at 1 mM
Pseudomonas aeruginosa
Mn2+
increases activity 27% at 1 mM
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-oxopropanoate + NAD+ + H2O
Pseudomonas aeruginosa
-
malonate + NADH
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.203
-
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified enzyme, 1 month
Pseudomonas aeruginosa
0°C, bovine serum albumin, NAD+, 1 week
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-oxopropanoate + NAD+ + H2O
-
348638
Pseudomonas aeruginosa
malonate + NADH
-
-
-
-
additional information
no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde
348638
Pseudomonas aeruginosa
?
-
-
-
-
NAD(P)+ + 3-oxopropanoate + H2O
-
348638
Pseudomonas aeruginosa
malonate + NAD(P)H
-
348638
Pseudomonas aeruginosa
ir
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
with NAD+ and bovine serum albumin at pH 8.7
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
-
malonate semialdehyde + NAD+
Pseudomonas aeruginosa
Other publictions for EC 1.2.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684557
Yebra
Identification of a gene clust ...
Lactobacillus casei
Appl. Environ. Microbiol.
73
3850-3858
2007
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1
1
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1
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1
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1
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1
1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
348638
Nakamura
Studies on malonic semialdehyd ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
50
147-152
1961
-
-
-
-
-
2
10
2
-
3
-
1
-
1
-
-
1
-
-
-
1
2
3
-
-
-
1
-
1
-
-
2
3
-
-
-
-
-
2
-
-
2
-
10
3
2
-
3
-
1
-
-
-
1
-
-
1
2
3
-
-
-
1
-
1
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