Literature summary for 1.2.1.13 extracted from

Isupov, M.N.; Fleming, T.M.; Dalby, A.R.; Crowhurst, G.S.; Bourne, P.C.; Littlechild, J.A.
Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus (1999), J. Mol. Biol., 291, 651-660.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
determination of the crystal structure of the apoenzyme by multiple isomorphous replacement at 2.05 A resolution, hanging drop technique. The crystals belong to space group P4(1)2(1)2 or its enantiomorph with cell dimensions a = b = 102.3 A, c = 181.6 A, which contract upon cryocooling at 100 K to a = b = 101.6 A, c = 179.9 A. The asymmetric unit contains two subunits with a molecular mass of 37611 Da	Saccharolobus solfataricus

Crystallization (Comment)

Organism

determination of the crystal structure of the apoenzyme by multiple isomorphous replacement at 2.05 A resolution, hanging drop technique. The crystals belong to space group P4(1)2(1)2 or its enantiomorph with cell dimensions a = b = 102.3 A, c = 181.6 A, which contract upon cryocooling at 100 K to a = b = 101.6 A, c = 179.9 A. The asymmetric unit contains two subunits with a molecular mass of 37611 Da

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P39460	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
GAPDH	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	activity half-life: 17 h. The thermostability of the enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulfide bond	Saccharolobus solfataricus

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Release 2023.1 (January 2023)