Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
NADP+ | pH 7.5, 50°C | Saccharolobus solfataricus | |
2.2 | - |
NAD+ | pH 7.5, 50°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
x * 39500, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P39460 | - |
- |
Purification (Comment) | Organism |
---|---|
heat treatment at 80°C is an effective first step in the purification of these recombinant enzymes from extracts of the Escherichia coli host | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.97 | - |
pH 7.5, 50°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39500, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
glyceraldehyde-3-phosphate dehydrogenase | - |
Saccharolobus solfataricus |
GraP-DH | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half life: 17 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus | |
NADP+ | Vmax/Km for NADP+ is 33fold higher compared to Vmax/Km for NAD+ | Saccharolobus solfataricus |