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Literature summary for 1.2.1.13 extracted from

  • Erttmann, K.D.; Kleensang, A.; Schneider, E.; Hammerschmidt, S.; Buettner, D.W.; Gallin, M.
    Cloning, characterization and DNA immunization of an Onchocerca volvulus glyceraldehyde-3-phosphate dehydrogenase (Ov-GAPDH) (2005), Biochim. Biophys. Acta, 1741, 85-94.
    View publication on PubMed

Application

Application Comment Organism
medicine Onchocerca volvulus antigens possibly involved in protection against human onchocerciasis, GAPDH as a therapeutic target in helminth infections Onchocerca volvulus

Cloned(Commentary)

Cloned (Comment) Organism
into the pJC45Flag vector for expression in the Escherichia coli strain PAPlaclQ DE3, into the vaccination vector pcDNA3.1+ for transformation of DH5alpha cells Onchocerca volvulus

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus nucleus of hypodermis Onchocerca volvulus 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
calculated Onchocerca volvulus
38000
-
SDS-PAGE and immunoblot Onchocerca volvulus

Organism

Organism UniProt Comment Textmining
Onchocerca volvulus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+ chelate chromatography and pH-shift under denaturating conditions Onchocerca volvulus

Source Tissue

Source Tissue Comment Organism Textmining
hypodermis
-
Onchocerca volvulus
-
uterine epithelium
-
Onchocerca volvulus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
740
-
NADH-enzyme activity Onchocerca volvulus

Synonyms

Synonyms Comment Organism
GAPDH
-
Onchocerca volvulus
glyceraldehyde-3-phosphate dehydrogenase
-
Onchocerca volvulus
Ov-GAPDH
-
Onchocerca volvulus