Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,3-diphosphoglycerate | the role of the reduction of the enzyme in vivo is to increase its sensitivity towards the activator 1,3-bisphosphoglycerate. The actual activation and aggregation state of the enzyme in chloroplasts in light is regulated by the concentration of 1,3-diphosphoglycerate as activator in the stroma and its actual activity by the availability of 1,3-diphosphoglycerate as substrate | Spinacia oleracea | |
ATP | activation in absence of NAD+, no activation in presence of NAD+ | Spinacia oleracea | |
dithiothreitol | - |
Spinacia oleracea | |
NADP+ | activation in absence of NAD+, no activation in presence of NAD+ | Spinacia oleracea | |
NADPH | activation in absence of NAD+, no activation in presence of NAD+ | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
3-phospho-D-glyceroyl phosphate | with NADH as cofactor, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate | Spinacia oleracea | |
0.02 | - |
3-phospho-D-glyceroyl phosphate | with NADPH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate | Spinacia oleracea | |
0.06 | - |
NADPH | activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate | Spinacia oleracea | |
0.29 | - |
NADH | with NADH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Spinacia oleracea | |
NADP+ | cofactor | Spinacia oleracea |