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Literature summary for 1.2.1.13 extracted from

  • Baalmann, E.; Backhausen, J.E.; Rak, C.; Vetter, S.; Scheibe, R.
    Reductive modification and nonreductive activation of purified spinach chloroplast NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (1995), Arch. Biochem. Biophys., 324, 201-208.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,3-diphosphoglycerate the role of the reduction of the enzyme in vivo is to increase its sensitivity towards the activator 1,3-bisphosphoglycerate. The actual activation and aggregation state of the enzyme in chloroplasts in light is regulated by the concentration of 1,3-diphosphoglycerate as activator in the stroma and its actual activity by the availability of 1,3-diphosphoglycerate as substrate Spinacia oleracea
ATP activation in absence of NAD+, no activation in presence of NAD+ Spinacia oleracea
dithiothreitol
-
Spinacia oleracea
NADP+ activation in absence of NAD+, no activation in presence of NAD+ Spinacia oleracea
NADPH activation in absence of NAD+, no activation in presence of NAD+ Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
3-phospho-D-glyceroyl phosphate with NADH as cofactor, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate Spinacia oleracea
0.02
-
3-phospho-D-glyceroyl phosphate with NADPH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate Spinacia oleracea
0.06
-
NADPH activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate Spinacia oleracea
0.29
-
NADH with NADH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADH
-
r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADPH
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Spinacia oleracea
NADP+ cofactor Spinacia oleracea